Clinic of Dentistry, St. Anne's Faculty Hospital, Pekarska 53, 656 91, Brno, Czech Republic.
Faculty of Medicine, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021 Jun;165(2):216-223. doi: 10.5507/bp.2020.003. Epub 2020 Feb 11.
Granulation tissue (GT) and specialized pro‑resolving mediators such as lipoxins and resolvins are key elements in the successful resolution of periodontitis. Aspirin‑triggered lipoxins and resolvins are even more powerful than their natural analogues. Their biosynthesis can be accelerated by omega-3 fatty acids. The aim of this study was to evaluate the use of GT enriched by aspirin and omega-3 fatty acids during the surgical treatment of periodontitis in an experimental animal model (rabbit).
In each of 24 rabbits, two experimental periodontal defects were created. In total, 47 defects were treated with open-flap debridement and one of three procedures: (1) GT extracted and soaked with aspirin and omega-3 fatty acids (ASA+OMEGA3 group); (2) GT soaked with saline (PLACEBO group); or (3) GT left untreated (CONTROL group). Then, the GT was replaced in situ. Primary evaluated criteria were the probing pocket depth (PPD) and the clinical attachment level (CAL). Necropsies were harvested 2, 6, and 12 weeks after surgery. The samples were used for histological and molecular biological assessment.
A trend of greater PPD and CAL in the ASA+OMEGA3 group was observed at 6 weeks. However, there was no significant difference between them. During the observation period, tissue levels of FGF-7, IL-1β and TIMP-1 showed a statistically significant decrease (P<0.05). For the other variables, the ASA+OMEGA3 group was comparable with the PLACEBO and CONTROL groups.
This experiment did not demonstrate the superiority of the proposed approach. However, the enriched granulation tissue did not impair healing outcomes.
肉芽组织（GT）和专门的促解决介质，如脂氧素和 resolvins，是成功解决牙周炎的关键因素。阿司匹林触发的脂氧素和 resolvins比它们的天然类似物更有效。它们的生物合成可以通过 ω-3 脂肪酸加速。本研究旨在评估在实验动物模型（兔）中使用富含阿司匹林和 ω-3 脂肪酸的 GT 进行牙周炎的手术治疗。
在 24 只兔子中，每只兔子都创建了两个实验性牙周缺损。总共 47 个缺陷用开放式瓣清创术治疗，并采用三种方法之一进行治疗：（1）提取 GT 并用阿司匹林和 ω-3 脂肪酸浸泡（ASA+OMEGA3 组）；（2）用生理盐水浸泡 GT（PLACEBO 组）；或（3）GT 未处理（CONTROL 组）。然后，GT 原位替换。主要评估标准是探诊袋深度（PPD）和临床附着水平（CAL）。手术后 2、6 和 12 周进行尸检。样本用于组织学和分子生物学评估。
在第 6 周时，ASA+OMEGA3 组的 PPD 和 CAL 呈增大趋势。然而，它们之间没有显著差异。在观察期间，FGF-7、IL-1β 和 TIMP-1 的组织水平呈统计学显著下降（P<0.05）。对于其他变量，ASA+OMEGA3 组与 PLACEBO 和 CONTROL 组相当。
本实验并未证明所提出方法的优越性。然而，富含的肉芽组织并未损害愈合结果。
