Dogan Guvenc, Karaca Onur
Hitit University School of Medicine, Department of Anesthesiology and Reanimation, Çorum, Turkey.
Turk Neurosurg. 2020;30(2):237-243. doi: 10.5137/1019-5149.JTN.27680-19.2.
To examine the effect of sevoflurane, a halogenated anesthetic used in clinical applications, on oxidative stress and inflammation after an acute traumatic brain injury (TBI) in rats.
Thirty male Spragueâ€"Dawley rats were divided into three groups: control (Group 1), trauma (Group 2), and trauma+sevoflurane (Group 3). A diffuse TBI model was created for Groups 2 and 3. Sevoflurane anesthesia was applied 6 hours a day after induced trauma in Group 3. Glutathione (GSH), malondialdehyde (MDA), and tissue myeloperoxidase (MPO) activities were measured in the blood. Tumor necrosis factor alpha (TNF-±), vascular endothelial growth factor (VEGF), and Bax primary antibodies were used to determine the effects of TBI.
MDA was significantly higher in Group 2 than in Group 1. There was a significant increase in tissue MPO levels in Groups 2 and 3 compared with those in Group 1. GSH levels decreased in Groups 2 and 3. Group 3 revealed degenerative changes in neurons and glial cells, vascular enlargement and congestion, and inflammatory cell infiltration around blood vessels. In Group 3, VEGF expression was positive in endothelial and inflammatory cells around blood vessels. Group 3 had positive TNF-± expression in neurons, small granular cells, and glial cells around blood vessels.
Sevoflurane administration in acute TBI did not prevent the development of oxidative stress and inflammation.
研究临床应用的卤化麻醉剂七氟醚对大鼠急性创伤性脑损伤（TBI）后氧化应激和炎症的影响。
将30只雄性Sprague-Dawley大鼠分为三组：对照组（第1组）、创伤组（第2组）和创伤+七氟醚组（第3组）。为第2组和第3组建立弥漫性TBI模型。第3组在创伤诱导后每天给予6小时的七氟醚麻醉。检测血液中的谷胱甘肽（GSH）、丙二醛（MDA）和组织髓过氧化物酶（MPO）活性。使用肿瘤坏死因子α（TNF-α）、血管内皮生长因子（VEGF）和Bax一抗来确定TBI的影响。
第2组的MDA显著高于第1组。与第1组相比，第2组和第3组的组织MPO水平显著升高。第2组和第3组的GSH水平降低。第3组显示神经元和神经胶质细胞有退行性改变、血管扩张和充血以及血管周围有炎性细胞浸润。在第3组中，血管周围的内皮细胞和炎性细胞中VEGF表达呈阳性。第3组中血管周围的神经元、小颗粒细胞和神经胶质细胞中TNF-α表达呈阳性。
急性TBI时给予七氟醚不能预防氧化应激和炎症的发生。
