Meshram G P, Rao K M
Division of Microbiology, Defence Research and Development Establishment, Gwalior, India.
Food Chem Toxicol. 1988 Sep;26(9):791-6. doi: 10.1016/0278-6915(88)90215-3.
N,N-diethylphenylacetamide (DEPA), a promising new insect repellent, was tested for mutagenicity in the in vitro Ames Salmonella/microsome mutagenicity test and the in vivo mouse micronucleus test. For the Ames test, DEPA was assayed both in the presence and absence of Aroclor 1254-induced rat-liver S-9 mix (5 and 20% S-9 fraction), using five tester strains of Salmonella typhimurium--TA97a, TA98, TA100, TA102 and TA104. For the micronucleus test, mice were exposed to DEPA through ip injection for 2 and 5 days in separate experiments, and bone marrow and peripheral blood were sampled 6 and 48 hr after the final injection, respectively. DEPA did not induce a mutagenic response in the Ames test, and mouse bone marrow and peripheral blood micronucleus tests. DEPA was not considered cytotoxic, as a depression of the percentage PCE was not observed at any dose in the range of 1 to 100 mg/kg body weight with either treatment protocol of the micronucleus test.
N,N - 二乙基苯乙酰胺（DEPA）是一种很有前景的新型驱虫剂，在体外艾姆斯沙门氏菌/微粒体诱变试验和体内小鼠微核试验中对其致突变性进行了检测。在艾姆斯试验中，使用鼠伤寒沙门氏菌的五个测试菌株——TA97a、TA98、TA100、TA102和TA104，在有和没有艾氏剂1254诱导的大鼠肝脏S - 9混合物（5%和20% S - 9组分）存在的情况下对DEPA进行了测定。在微核试验中，在单独的实验中通过腹腔注射使小鼠接触DEPA 2天和5天，分别在最后一次注射后6小时和48小时采集骨髓和外周血。DEPA在艾姆斯试验以及小鼠骨髓和外周血微核试验中均未诱导出诱变反应。在微核试验的任何一种处理方案中，在体重1至100 mg/kg范围内的任何剂量下均未观察到嗜多染红细胞百分比降低，因此DEPA不被认为具有细胞毒性。
