Bhattacharyya A, Yanagimachi R
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822.
Gamete Res. 1988 Feb;19(2):123-9. doi: 10.1002/mrd.1120190203.
Bicarbonate ions (HCO3-) in the medium are not absolutely essential for the fertilization of guinea pig eggs. However, fertilization takes place most efficiently in HCO3- -buffered medium. Capacitation, the acrosome reaction, and zona penetration by spermatozoa can occur in HCO3- -free media with synthetic organic buffers (i.e., MOPS, TES, HEPES, Tris, and TAPSO) but not as efficiently as in the HCO3- -buffered medium. It appears that HCO3- functions as more than just a pH-buffering molecule.
培养基中的碳酸氢根离子（HCO3-）对于豚鼠卵子的受精并非绝对必要。然而，在HCO3-缓冲培养基中受精效率最高。精子的获能、顶体反应和穿过透明带在含有合成有机缓冲剂（即MOPS、TES、HEPES、Tris和TAPSO）的无HCO3-培养基中可以发生，但效率不如在HCO3-缓冲培养基中高。看来HCO3-的作用不止是一个pH缓冲分子。
