College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.
Management Office of Caozhou Peony Garden, Heze 274000, Shandong, China.
Genes (Basel). 2020 Feb 19;11(2):214. doi: 10.3390/genes11020214.
The insufficient number of available simple sequence repeats (SSRs) inhibits genetic research on and molecular breeding of , a flowering crop with great economic value. The objective of this study was to develop SSRs for with Illumina RNA sequencing and assess the role of SSRs in gene regulation. The results showed that dinucleotides with AG/CT repeats were the most abundant type of repeat motif in and were preferentially distributed in untranslated regions. Significant differences in SSR size were observed among motif types and locations. A large number of unigenes containing SSRs participated in catalytic activity, metabolic processes and cellular processes, and 28.16% of all transcription factors and 21.74% of hub genes for inflorescence stem straightness were found to contain SSRs. Successful amplification was achieved with 89.05% of 960 pairs of SSR primers, 55.83% of which were polymorphic, and most of the 46 tested primers had a high level of transferability to the genus . Principal component and cluster dendrogram analyses produced results consistent with known genealogical relationships. This study provides a set of SSRs with abundant information for future accession identification, marker-trait association and molecular assisted breeding in .
可用简单重复序列 (SSR) 的数量不足，限制了具有巨大经济价值的花卉作物 的遗传研究和分子育种。本研究旨在利用 Illumina RNA 测序为 开发 SSR，并评估 SSR 在基因调控中的作用。结果表明，在 中，二核苷酸重复基序 AG/CT 是最丰富的重复基序类型，并且优先分布在非翻译区。在基序类型和位置之间观察到 SSR 大小的显著差异。大量含有 SSR 的基因座参与催化活性、代谢过程和细胞过程，并且发现 28.16%的所有转录因子和花序茎直度的 21.74%的枢纽基因都含有 SSR。成功扩增了 960 对 SSR 引物中的 89.05%，其中 55.83%具有多态性，并且 46 个测试引物中的大多数具有向属转移的高水平。主成分和聚类树状图分析结果与已知的系统发育关系一致。本研究为未来的品种鉴定、标记-性状关联和分子辅助育种提供了一套信息丰富的 SSR。
