Transcriptome Analysis of Two Different Developmental Stages of Seeds.

is a herbaceous flower in the family with both hypocotyl and epicotyl dormant seeds. We used high-throughput transcriptome sequencing on two different developmental stages of seeds to identify seed dormancy and germination-related genes. We performed de novo assembly and annotated a total of 123,577 unigenes, which encoded 24,688 putative proteins with 47 GO categories. A total of 10,714 unigenes were annotated in the KEGG database, and 258 pathways were involved in the annotations. A total of 1795 genes were differentially expressed in the functional enrichment analysis. The key genes for seed germination and dormancy, such as and , were confirmed by quantitative reverse transcription-polymerase chain reaction analysis. This is the first report of sequencing the seed transcriptome. Our results provide fundamental frame work and technical support for further selective breeding and cultivation of . Our transcriptomic data also serves as the basis for future genetics and genomics research on and its closely related species.