Ma Yonglei, Cui Jinqiu, Lu Xiujun, Zhang Lijie, Chen Zhijing, Fei Riwen, Sun Xiaomei
Forestry College, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.
Int J Genomics. 2017;2017:8027626. doi: 10.1155/2017/8027626. Epub 2017 Aug 7.
is a herbaceous flower in the family with both hypocotyl and epicotyl dormant seeds. We used high-throughput transcriptome sequencing on two different developmental stages of seeds to identify seed dormancy and germination-related genes. We performed de novo assembly and annotated a total of 123,577 unigenes, which encoded 24,688 putative proteins with 47 GO categories. A total of 10,714 unigenes were annotated in the KEGG database, and 258 pathways were involved in the annotations. A total of 1795 genes were differentially expressed in the functional enrichment analysis. The key genes for seed germination and dormancy, such as and , were confirmed by quantitative reverse transcription-polymerase chain reaction analysis. This is the first report of sequencing the seed transcriptome. Our results provide fundamental frame work and technical support for further selective breeding and cultivation of . Our transcriptomic data also serves as the basis for future genetics and genomics research on and its closely related species.
是一种隶属于该科的草本花卉，其种子的下胚轴和上胚轴均处于休眠状态。我们对种子的两个不同发育阶段进行了高通量转录组测序，以鉴定与种子休眠和萌发相关的基因。我们进行了从头组装，共注释了123,577个单基因，这些单基因编码了具有47个基因本体（GO）类别的24,688个推定蛋白质。共有10,714个单基因在KEGG数据库中得到注释，且注释涉及258条通路。在功能富集分析中共有1795个基因差异表达。通过定量逆转录-聚合酶链反应分析证实了种子萌发和休眠的关键基因，如和。这是对种子转录组进行测序的首次报道。我们的结果为进一步对进行选择性育种和栽培提供了基础框架和技术支持。我们的转录组数据也为未来对及其近缘物种的遗传学和基因组学研究奠定了基础。
