Ibrahimi Manar, Korichi Wassila, Hafidi Mohamed, Lemee Laurent, Ouhdouch Yedir, Loqman Souad
Laboratory of Microbial Biotechnologies, Agrosciences and Environment (BioMAgE), Faculty of Sciences Semlalia, Cadi Ayyad University, PO Box 2390, Marrakesh, Morocco.
Institut de Chimie des Milieux et Matériaux de Poitiers (IC2MP - CNRS UMR 7285), Université de Poitiers, 4 rue Michel Brunet - TSA 51106, 86073 Poitiers Cedex 9, France.
Antibiotics (Basel). 2020 Feb 19;9(2):91. doi: 10.3390/antibiotics9020091.
Predatory bacteria constitute a heterogeneous group of prokaryotes able to lyse and feed on the cellular constituents of other bacteria in conditions of nutrient scarcity. In this study, we describe the isolation of Actinobacteria predator of other bacteria from the marine water of the Moroccan Atlantic coast. Only 4 Actinobacteria isolates showing strong predation capability against native or multidrug-resistant Gram-positive or Gram-negative bacteria were identified among 142 isolated potential predatory bacteria. These actinobacterial predators were shown to belong to the genus and to inhibit the growth of various native or multidrug-resistant micro-organisms, including , (native and methicillin-resistant), and (native and ampicillin-resistant). Even if no clear correlation could be established between the antibacterial activities of the selected predator Actinobacteria and their predatory activity, we cannot exclude that some specific bio-active secondary metabolites were produced in this context and contributed to the killing and lysis of the bacteria. Indeed, the co-cultivation of Actinobacteria with other bacteria is known to lead to the production of compounds that are not produced in monoculture. Furthermore, the production of specific antibiotics is linked to the composition of the growth media that, in our co-culture conditions, exclusively consisted of the components of the prey living cells. Interestingly, our strategy led to the isolation of bacteria with interesting inhibitory activity against methicillin-resistant (MRSA) as well as against Gram-negative bacteria.
掠食性细菌是一类异质的原核生物，在营养匮乏的条件下能够裂解并以其他细菌的细胞成分作为食物。在本研究中，我们描述了从摩洛哥大西洋沿岸海水中分离出的捕食其他细菌的放线菌。在142株分离出的潜在掠食性细菌中，仅鉴定出4株对天然或耐多药革兰氏阳性或革兰氏阴性细菌具有强捕食能力的放线菌分离株。这些放线菌掠食者被证明属于该属，并能抑制各种天然或耐多药微生物的生长，包括金黄色葡萄球菌（天然和耐甲氧西林的）以及大肠杆菌（天然和耐氨苄青霉素的）。即使在所选择的掠食性放线菌的抗菌活性与其捕食活性之间无法建立明确的相关性，但我们不能排除在这种情况下产生了一些特定的生物活性次级代谢产物，并有助于细菌的杀灭和裂解。事实上，已知放线菌与其他细菌共培养会导致产生在单培养中不产生的化合物。此外，特定抗生素的产生与生长培养基的组成有关，在我们的共培养条件下，生长培养基仅由猎物活细胞的成分组成。有趣的是，我们的策略导致分离出了对耐甲氧西林金黄色葡萄球菌（MRSA）以及革兰氏阴性细菌具有有趣抑制活性的细菌。
