预变性浓度的胍诱导的蛋白质构象变化。

Protein conformational changes induced by guanidine at predenaturational concentrations.

作者信息

Bismuto E, Irace G

机构信息

Section for Chemistry and Biochemistry, Faculty of Medicine, University of Naples, Italy.

出版信息

Int J Pept Protein Res. 1988 Nov;32(5):321-5. doi: 10.1111/j.1399-3011.1988.tb01266.x.

DOI:10.1111/j.1399-3011.1988.tb01266.x

PMID:3209352

Abstract

The nature of the molecular event that apomyoglobin undergoes at predenaturational concentrations of guanidine has been investigated by means of steady-state and multifrequency phase and modulation fluorometry. The results have been compared to those observed for liver alcohol dehydrogenase. From these studies has been hypothesized a different susceptibility of the distinct elements of secondary, super-secondary, and tertiary structure towards the denaturing action of guanidine at predenaturational concentrations.

摘要

通过稳态和多频相位及调制荧光法研究了脱辅基肌红蛋白在变性前浓度的胍中所经历的分子事件的性质。已将结果与观察到的肝醇脱氢酶的结果进行了比较。从这些研究中推测，二级、超二级和三级结构的不同元素对变性前浓度的胍的变性作用具有不同的敏感性。