日本质子加速器研究机构中微子蛋白质晶体学的现状与近期计划。
Current status and near future plan of neutron protein crystallography at J-PARC.
机构信息
Domain of Materials Science and Engineering, Graduate School of Science and Engineering, Ibaraki University, Hitachi, Ibaraki, Japan; Divisions of Neutron Beamline and Research, Frontier Research Center for Applied Atomic Sciences, Tokai, Ibaraki, Japan.
Institute for Integrated Radiation and Nuclear Science, Kyoto University, Kumatori, Osaka, Japan.
出版信息
Methods Enzymol. 2020;634:101-123. doi: 10.1016/bs.mie.2020.01.002. Epub 2020 Feb 8.
The IBARAKI Biological Crystal Diffractometer (iBIX) has been available for use at MLF (Material and Life Science Facility) in J-PARC (Japan Proton Accelerator Research Complex) since 2008. The development in state-of-the-art detector systems could enable iBIX to become one of the highest-performance neutron single-crystal diffractometers in the world. Here, together with other various developments, such as data reduction software, crystal growth, and new techniques in measurement coupled analysis, we provided new hydrogen and water structural data of several proteins and macromolecules. Although the proton power at MLF has not yet reached its planned maximum (1MW), a more powerful neutron source will be soon needed for neutron protein crystallography. A future idea is also proposed and discussed in this article.
自 2008 年以来，茨城县东海村的日本质子加速器研究设施（J-PARC）的材料和生命科学设施（MLF）就已经可以使用茨城生物晶体衍射仪（iBIX）了。最先进的探测器系统的发展使 iBIX 有望成为世界上性能最高的中子单晶衍射仪之一。在这里，我们与其他各种发展相结合，例如数据还原软件、晶体生长以及测量耦合分析中的新技术，提供了几种蛋白质和大分子的新的氢和水结构数据。尽管 MLF 的质子功率尚未达到计划的最大值（1MW），但对于中子蛋白质晶体学来说，很快就需要更强大的中子源。本文还提出并讨论了未来的想法。
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