IL-17 产生的ɑβT 和 γδT 细胞在卵巢癌患者中的意义。

Implication of IL-17 producing ɑβT and γδT cells in patients with ovarian cancer.

机构信息

Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, 210029 Nanjing, China; National Key Clinical Department of Laboratory Medicine, 210029 Nanjing, China.

出版信息

Hum Immunol. 2020 May;81(5):244-248. doi: 10.1016/j.humimm.2020.02.002. Epub 2020 Feb 21.

DOI:10.1016/j.humimm.2020.02.002

PMID:32093885

Abstract

OBJECTIVE

To investigate the expression and cellular source of IL-17A in human ovarian cancer (OC), benign ovarian tumor (BOT) and borderline ovarian tumor (OBT).

METHODS

RT-PCR and immunohistochemistry were used to measure the expression level of IL-17A in human OC tissues. Find concrete source of the elevated IL-17A levels in OC tissues by flow cytometry.

RESULTS

We found that IL-17A is expressed at higher levels in OC tissues than in BOT or OBT tissues at both the mRNA and protein levels. Moreover, high tumor IL-17A expression was significantly associated with poor tumor differentiation and positive lymph node status. Flow cytometric analysis demonstrated that significantly higher proportions of tumor-infiltrating IL-17A-producing CD4 T cells (Th17), CD8 T cells (Tc17), and γδT cells (IL-17 γδT) were present in OC tissue compared with BOT tissue. Of these, tumor-infiltrating γδT cells were the predominant source of IL-17A in OC and BOT patients. Finally, we found that the abundance of tumor-infiltrating IL-17 γδT cells, but not Th17 or Tc17 cells, was positively correlated with larger tumor size and lymph node metastasis in patients with advanced OC. These data suggest that increased tumor-infiltrating IL-17 γδ T cells may be associated with cancer progression in OC patients.

摘要

目的

研究白细胞介素-17A（IL-17A）在人卵巢癌（OC）、良性卵巢肿瘤（BOT）和交界性卵巢肿瘤（OBT）中的表达和细胞来源。

方法

采用 RT-PCR 和免疫组织化学方法检测人 OC 组织中 IL-17A 的表达水平。通过流式细胞术寻找 OC 组织中升高的 IL-17A 水平的具体来源。

结果

我们发现，在 OC 组织中，IL-17A 在 mRNA 和蛋白水平上的表达均高于 BOT 或 OBT 组织。此外，高肿瘤 IL-17A 表达与肿瘤分化不良和淋巴结阳性状态显著相关。流式细胞术分析表明，OC 组织中浸润的 IL-17A 产生 CD4 T 细胞（Th17）、CD8 T 细胞（Tc17）和 γδT 细胞（IL-17 γδT）的比例明显高于 BOT 组织。其中，浸润的 γδT 细胞是 OC 和 BOT 患者中 IL-17A 的主要来源。最后，我们发现，浸润的 IL-17 γδT 细胞的丰度，而不是 Th17 或 Tc17 细胞，与晚期 OC 患者的肿瘤体积较大和淋巴结转移呈正相关。这些数据表明，肿瘤浸润的 IL-17 γδT 细胞的增加可能与 OC 患者的癌症进展有关。