使用微光学透镜阵列对液滴中单细胞β-半乳糖苷酶活性进行超高通量检测。
Ultra-high throughput detection of single cell -galactosidase activity in droplets using micro-optical lens array.
作者信息
Lim Jiseok, Vrignon Jérémy, Gruner Philipp, Karamitros Christos S, Konrad Manfred, Baret Jean-Christophe
机构信息
Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, D-37077 Goettingen, Germany.
Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Goettingen, Germany.
出版信息
Appl Phys Lett. 2013 Nov 11;103(20):203704. doi: 10.1063/1.4830046. Epub 2013 Nov 14.
Abstract
We demonstrate the use of a hybrid microfluidic-micro-optical system for the screening of enzymatic activity at the single cell level. β-galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets containing cells are screened by measuring their fluorescence signal using a high-speed camera. The measurement is parallelized over 100 channels equipped with microlenses and analyzed by image processing. A reinjection rate of 1 ml of emulsion per minute was reached corresponding to more than 10 droplets per second, an analytical throughput larger than those obtained using flow cytometry.
摘要
我们展示了一种用于单细胞水平酶活性筛选的混合微流控-微光学系统。通过荧光测定法在100皮升液滴中揭示β-半乳糖苷酶活性。通过使用高速相机测量含有细胞的单个液滴的荧光信号来进行筛选。测量在配备微透镜的100个通道上并行进行，并通过图像处理进行分析。达到了每分钟1毫升乳液的再注入速率，相当于每秒超过10个液滴，分析通量高于使用流式细胞术获得的通量。
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