Zinati-Saeed Sakineh, Shakiba Ebrahim, Rahimi Ziba, Akbari Mona, Najafi Fariba, Bahrehmand Fariborz, Vaisi-Raygani Asad, Rahimi Zohreh, Ebrahimi Ali, Rahimi Mehrali
Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Medical Biology Research Center, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Iran J Pathol. 2020 Winter;15(1):23-29. doi: 10.30699/IJP.2019.105695.2098.
BACKGROUND & OBJECTIVE: To find an association between gene variants of insulin-like growth factor-1 (IGF-1) and 5,10-methylenetetrahydrofolate reductase (MTHFR) with the risk of acne vulgaris (AV).
In a case-control study, we investigated 150 AV patients and 148 healthy individuals (aged 18-25 years) for the IGF-1 G>A and MTHFR C677T polymorphisms, as well as the serum levels of IGF-1, insulin, and the homeostasis model assessment of insulin resistance (HOMA-IR). The serum biochemical parameters and the genotypes of IGF-1 G>A and MTHFR C677T were detected by using appropriate kits and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods, respectively.
The frequencies of IGF-1 and the MTHFR polymorphisms were not significantly different comparing patients and controls. The serum level of IGF-1 was 179.8±72.8 µg/L in AV patients compared to 164.6±63.7 µg/L in controls (=0.056). The serum level of insulin in female patients was significantly higher than controls. The HOMA was 3.54±5.6 in patients compared to 1.16±1.4 (<0.001) in controls. Significantly higher levels of fasting blood sugar (FBS), total cholesterol, and low-density lipoprotein-cholesterol (LDL-C) were detected in female patients than controls. However, the level of estradiol was significantly lower in female patients than in controls. In females, the presence of the MTHFR T allele was associated with significantly higher levels of FBS and LDL-C, as well as a significantly lower level of estradiol compared to those carriers of the C allele.
We found the absence of an association between IGF-1 and MTHFR polymorphisms with the risk of AV. However, increased insulin, IGF-1, and HOMA levels in AV patients indicated the effect of insulin and insulin resistance in the risk of AV and its severity.
探究胰岛素样生长因子-1（IGF-1）和5,10-亚甲基四氢叶酸还原酶（MTHFR）基因变异与寻常痤疮（AV）风险之间的关联。
在一项病例对照研究中，我们调查了150例AV患者和148名健康个体（年龄在18 - 25岁之间）的IGF-1 G>A和MTHFR C677T基因多态性，以及IGF-1、胰岛素的血清水平和胰岛素抵抗的稳态模型评估（HOMA-IR）。分别使用合适的试剂盒和聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测血清生化参数以及IGF-1 G>A和MTHFR C677T的基因型。
比较患者和对照组，IGF-1和MTHFR基因多态性的频率无显著差异。AV患者的IGF-1血清水平为179.8±72.8μg/L，而对照组为164.6±63.7μg/L（P = 0.056）。女性患者的胰岛素血清水平显著高于对照组。患者的HOMA为3.54±5.6，而对照组为1.16±1.4（P<0.001）。女性患者的空腹血糖（FBS）、总胆固醇和低密度脂蛋白胆固醇（LDL-C）水平显著高于对照组。然而，女性患者的雌二醇水平显著低于对照组。在女性中，与C等位基因携带者相比，MTHFR T等位基因的存在与显著更高的FBS和LDL-C水平以及显著更低的雌二醇水平相关。
我们发现IGF-1和MTHFR基因多态性与AV风险之间不存在关联。然而，AV患者中胰岛素、IGF-1和HOMA水平升高表明胰岛素和胰岛素抵抗对AV风险及其严重程度有影响。
