Department of Medical Physics, University of Wisconsin - Madison, Madison WI USA.
Department of Biomedical Engineering, Tufts School of Engineering, Medford MA USA.
Biomed Phys Eng Express. 2020 Jan;6(1). doi: 10.1088/2057-1976/ab6e16. Epub 2020 Jan 31.
Understanding the dynamic nature of tumor hypoxia is vital for cancer therapy. The presence of oxygen within a tumor during radiation therapy increases the likelihood of local control. We used a novel interstitial diffuse optical probe to make real-time measurements of blood volume fraction and hemoglobin oxygen saturation within a tumor at a high temporal resolution. This device was initially characterized and benchmarked using a customized vessel designed to control hemoglobin oxygen saturation and blood volume in a solution of blood with different concentrations of an oxygen scavenger, tetrakis (hydroxymethyl) phosphonium chloride. The optical device was found to consistently monitor the changes in oxygen saturation and these changes correlated to the concentration of the oxygen scavenger added. In near-simultaneous measurements of blood volume and oxygen saturation in tumor-bearing mice, the changes in blood volume fraction and oxygen saturation measured with the interstitial diffuse optical probe were benchmarked against photoacoustic imaging system to track and compare temporal dynamics of oxygen saturation and blood volume in a patient-derived xenograft model of hypopharyngeal carcinoma. Positive correlations between our device and photoacoustic imaging in measuring blood volume and oxygen saturation were observed.
了解肿瘤缺氧的动态变化对于癌症治疗至关重要。在放射治疗过程中，肿瘤内存在氧气会增加局部控制的可能性。我们使用一种新型的间质漫反射光学探头，以高时间分辨率实时测量肿瘤内的血容量分数和血红蛋白氧饱和度。该设备最初使用一种定制的血管进行了表征和基准测试，该血管设计用于控制血红蛋白氧饱和度和不同浓度氧清除剂四羟甲基氯化磷在血液溶液中的血容量。该光学设备能够持续监测氧饱和度的变化，并且这些变化与添加的氧清除剂浓度相关。在荷瘤小鼠的血容量和氧饱和度的近同步测量中，间质漫反射光学探头测量的血容量分数和氧饱和度的变化与光声成像系统进行了基准测试，以跟踪和比较缺氧型下咽癌患者来源异种移植模型中氧饱和度和血容量的时间动态。我们的设备与光声成像在测量血容量和氧饱和度方面表现出正相关。
