Vu Quang Huy, Tran Diep Tuan, Tran Phu Manh Sieu, Le Van Chuong, Huynh Thi Diem Phuc, Bui Quang Sang
University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam.
University Medical Center Ho Chi Minh City, Vietnam.
J Parasitol Res. 2020 Feb 10;2020:6842975. doi: 10.1155/2020/6842975. eCollection 2020.
External quality assessment (EQA) provides evidence of reliable, accurate, and precise results for customers using the diagnostic test for . To establish a procedure for producing standard serum samples for serodiagnostic testing in EQA.
The collected serum samples to contain anti- serum samples for serodiagnostic testing in EQA. -test and a -test were applied to evaluate their homogeneity and stability.
Among eleven samples positive by ELISA, ten of them were confirmed via Western blotting by positive reaction with 5 specific serum samples for serodiagnostic testing in EQA. serum samples for serodiagnostic testing in EQA. > 0.05). Samples produced by both methods were stable for 7 days at 30°C ( > 0.05). Samples produced by both methods were stable for 7 days at 30°C (.
Specific serodiagnosis samples of anti- antibodies for EQA could be produced that possessed homogeneity and stability lasting for 3 months and 6 months by the freeze-drying and freezing methods, respectively. At 30°C, the samples produced by both methods were stable for 7 days, suitable for delivery to remote laboratories. serum samples for serodiagnostic testing in EQA.
外部质量评估（EQA）为使用诊断测试的客户提供可靠、准确和精确结果的证据。建立一种在EQA中生产用于血清学诊断测试的标准血清样本的程序。
收集的血清样本包含用于EQA中血清学诊断测试的抗血清样本。应用 - 测试和 - 测试来评估它们的均匀性和稳定性。
在通过酶联免疫吸附测定（ELISA）呈阳性的11个样本中，其中10个通过蛋白质印迹法与5个用于EQA中血清学诊断测试的特异性血清样本呈阳性反应得到确认。用于EQA中血清学诊断测试的血清样本。> 0.05）。两种方法生产的样本在30°C下7天内稳定（> 0.05）。两种方法生产的样本在30°C下7天内稳定（.
通过冷冻干燥和冷冻方法可分别生产出用于EQA的抗抗体特异性血清学诊断样本，其均匀性和稳定性分别持续3个月和6个月。在30°C下，两种方法生产的样本7天内稳定，适合运送到偏远实验室。用于EQA中血清学诊断测试的血清样本。
