Ponomareva Tatyana, Sliadovskii Dmitrii, Timchenko Maria, Molchanov Maxim, Timchenko Alexander, Sogorin Evgeny
Federal Research Center "Pushchino Scientific Center for Biological Research of the RAS", Pushchino, Russia.
Institute of Theoretical and Experimental Biophysics of the RAS, Pushchino, Russia.
PeerJ. 2020 Feb 12;8:e8579. doi: 10.7717/peerj.8579. eCollection 2020.
In this study, several methods were used to analyze the hydrolysis of hyaluronic acid (HA)-based cosmetic fillers by the hepatopancreas homogenate of the Red king crab. The results show that the homogenate and commercially available hyaluronidases have similar hydrolysis activities on the fillers. Atomic force microscopy images reveal that the HA fillers consist mainly of spherical-like particles, which are converted into filamentous structures as a result of hydrolysis by the Red king crab hepatopancreas homogenate. Turbidimetric analysis of the hydrolysis process shows that HA aggregation with acidic albumin exhibits a bell-shaped dependence on reaction time. Analysis of the hydrolysis process by nuclear magnetic resonance shows that HA degradation lasts several days. The maximum rate of the reaction is detected in the 1st h of incubation. The data confirm that the purified homogenate of the Red king crab hepatopancreas exerts hyaluronidase activity on HA-based cosmetic fillers; therefore, it may be considered as a potential therapeutic agent for treating filler complications.
在本研究中，采用了几种方法来分析红王蟹肝胰腺匀浆对基于透明质酸（HA）的美容填充剂的水解作用。结果表明，该匀浆与市售透明质酸酶对填充剂具有相似的水解活性。原子力显微镜图像显示，HA填充剂主要由球形颗粒组成，这些颗粒在红王蟹肝胰腺匀浆水解后转化为丝状结构。水解过程的比浊分析表明，HA与酸性白蛋白的聚集对反应时间呈钟形依赖关系。核磁共振对水解过程的分析表明，HA降解持续数天。在孵育的第1小时检测到最大反应速率。数据证实，红王蟹肝胰腺的纯化匀浆对基于HA的美容填充剂具有透明质酸酶活性；因此，它可被视为治疗填充剂并发症的潜在治疗剂。
