透明质酸的轻敲模式原子力显微镜：伸展且分子内相互作用的链。
Tapping mode atomic force microscopy of hyaluronan: extended and intramolecularly interacting chains.
作者信息
Cowman M K, Li M, Balazs E A
机构信息
Department of Chemical Engineering, Chemistry, and Materials Science and Herman F. Mark Polymer Research Institute, Polytechnic University, Brooklyn, New York 11201, USA.
出版信息
Biophys J. 1998 Oct;75(4):2030-7. doi: 10.1016/S0006-3495(98)77645-3.
Abstract
The extracellular matrix polysaccharide hyaluronan has been examined by tapping mode atomic force microscopy. High molecular weight hyaluronan was deposited on mica from dilute aqueous solution and imaged in air. Long unbranched chains could be observed and were found to be compatible with the known covalent structure of hyaluronan. In addition, chains with evidence of intramolecular association were observed. In the simplest cases, the association took the form of loops stabilized by antiparallel double-stranded (probably double-helical) segments. In other cases, the polarity of the associated regions could not be determined. Extensive intramolecular association in long hyaluronan chains resulted in a fenestrated structure of the same type as that formed by intermolecular association at higher concentrations.
摘要
已通过轻敲模式原子力显微镜对细胞外基质多糖透明质酸进行了研究。将高分子量透明质酸从稀水溶液沉积在云母上，并在空气中成像。可以观察到长的无分支链，并且发现其与已知的透明质酸共价结构相符。此外，还观察到有分子内缔合迹象的链。在最简单的情况下，缔合采取由反平行双链（可能是双螺旋）片段稳定的环的形式。在其他情况下，缔合区域的极性无法确定。长透明质酸链中广泛的分子内缔合导致形成了一种与在较高浓度下分子间缔合形成的结构相同类型的有孔结构。
相似文献
1
Tapping mode atomic force microscopy of hyaluronan: extended and intramolecularly interacting chains.透明质酸的轻敲模式原子力显微镜：伸展且分子内相互作用的链。
Biophys J. 1998 Oct;75(4):2030-7. doi: 10.1016/S0006-3495(98)77645-3.
2
Extended, relaxed, and condensed conformations of hyaluronan observed by atomic force microscopy.通过原子力显微镜观察到的透明质酸的伸展、松弛和凝聚构象。
Biophys J. 2005 Jan;88(1):590-602. doi: 10.1529/biophysj.104.049361. Epub 2004 Oct 15.
3
Hyaluronic acid by atomic force microscopy.通过原子力显微镜观察透明质酸。
J Struct Biol. 1999 Jun 1;126(1):52-8. doi: 10.1006/jsbi.1999.4090.
4
Hyaluronan conformations on surfaces: effect of surface charge and hydrophobicity.表面上的透明质酸构象：表面电荷和疏水性的影响。
Carbohydr Res. 2005 Apr 11;340(5):929-41. doi: 10.1016/j.carres.2005.01.024.
5
Observation of the helical structure of the bacterial polysaccharide acetan by atomic force microscopy.通过原子力显微镜观察细菌多糖乙酰聚糖的螺旋结构。
Biophys J. 1995 Jan;68(1):360-3. doi: 10.1016/S0006-3495(95)80195-5.
6
Tapping mode Atomic Force Microscopy of scleroglucan networks.硬葡聚糖网络的轻敲模式原子力显微镜观察
Biopolymers. 1997;42(1):89-100. doi: 10.1002/(SICI)1097-0282(199707)42:1<89::AID-BIP8>3.0.CO;2-Y.
7
Experimental approaches to hyaluronan structure.透明质酸结构的实验方法。
Carbohydr Res. 2005 Apr 11;340(5):791-809. doi: 10.1016/j.carres.2005.01.022.
8
Atomic force microscopy imaging of supramolecular organization of hyaluronan and its receptor CD44.原子力显微镜成像超分子组织的透明质酸及其受体 CD44。
IEEE Trans Nanobioscience. 2009 Dec;8(4):294-9. doi: 10.1109/TNB.2009.2035251.
9
Effect of molecular weight on the exponential growth and morphology of hyaluronan/chitosan multilayers: a surface plasmon resonance spectroscopy and atomic force microscopy investigation.分子量对透明质酸/壳聚糖多层膜指数生长及形态的影响：表面等离子体共振光谱和原子力显微镜研究
J Am Chem Soc. 2005 Jun 29;127(25):9224-34. doi: 10.1021/ja044385n.
10
Single-molecular enzymatic elongation of hyaluronan polymers visualized by high-speed atomic force microscopy.高速原子力显微镜可视化的透明质酸聚合物的单分子酶延伸。
J Am Chem Soc. 2012 Dec 19;134(50):20254-7. doi: 10.1021/ja309646s. Epub 2012 Dec 4.
引用本文的文献
1
Highly Branched Sulfated Glycopolymers as Mucin Mimetics.高度支化的硫酸化糖聚合物作为粘蛋白模拟物
J Am Chem Soc. 2025 Sep 10;147(36):32698-32709. doi: 10.1021/jacs.5c08232. Epub 2025 Aug 27.
2
The effect of hepatopancreas homogenate of the Red king crab on HA-based filler.红帝王蟹肝胰腺匀浆对透明质酸基填充剂的影响。
PeerJ. 2020 Feb 12;8:e8579. doi: 10.7717/peerj.8579. eCollection 2020.
3
The material properties of naked mole-rat hyaluronan.裸鼹鼠透明质酸的材料特性。
Sci Rep. 2019 Apr 29;9(1):6632. doi: 10.1038/s41598-019-43194-7.
4
What is special about 200 kDa hyaluronan that activates hyaluronan receptor signaling?激活透明质酸受体信号传导的200 kDa透明质酸有什么特别之处？
Glycobiology. 2017 Sep 1;27(9):868-877. doi: 10.1093/glycob/cwx039.
5
Nanomechanics of the Cartilage Extracellular Matrix.软骨细胞外基质的纳米力学
Annu Rev Mater Res. 2011 Jul 1;41:133-168. doi: 10.1146/annurev-matsci-062910-100431.
6
Aggrecan, an unusual polyelectrolyte: review of solution behavior and physiological implications.聚集蛋白聚糖，一种不寻常的聚电解质：溶液行为综述及其生理意义。
Acta Biomater. 2012 Jan;8(1):3-12. doi: 10.1016/j.actbio.2011.08.011. Epub 2011 Aug 17.
7
Adult bone marrow stromal cell-based tissue-engineered aggrecan exhibits ultrastructure and nanomechanical properties superior to native cartilage.基于成人骨髓基质细胞的组织工程化聚集蛋白聚糖具有优于天然软骨的超微结构和纳米力学性能。
Osteoarthritis Cartilage. 2010 Nov;18(11):1477-86. doi: 10.1016/j.joca.2010.07.015. Epub 2010 Aug 6.
8
Polypeptide grafted hyaluronan: synthesis and characterization.多肽接枝透明质酸：合成与表征。
Biomacromolecules. 2010 Sep 13;11(9):2313-20. doi: 10.1021/bm1004146.
9
Extended, relaxed, and condensed conformations of hyaluronan observed by atomic force microscopy.通过原子力显微镜观察到的透明质酸的伸展、松弛和凝聚构象。
Biophys J. 2005 Jan;88(1):590-602. doi: 10.1529/biophysj.104.049361. Epub 2004 Oct 15.
10
Organization and adhesive properties of the hyaluronan pericellular coat of chondrocytes and epithelial cells.软骨细胞和上皮细胞透明质酸细胞周被膜的组织与黏附特性。
Biophys J. 2003 Sep;85(3):1996-2005. doi: 10.1016/S0006-3495(03)74627-X.
本文引用的文献
1
Chemical structure of hyaluronic acid.透明质酸的化学结构。
Fed Proc. 1958 Dec;17(4):1075-7.
2
The structure and function of hyaluronan: An overview.透明质酸的结构与功能：概述
Immunol Cell Biol. 1996 Apr;74(2):A1-7. doi: 10.1038/icb.1996.32.
3
An agarose gel electrophoretic method for analysis of hyaluronan molecular weight distribution.一种用于分析透明质酸分子量分布的琼脂糖凝胶电泳方法。
Anal Biochem. 1994 Jun;219(2):278-87. doi: 10.1006/abio.1994.1267.
4
Conformation and dynamic interactions in hyaluronate solutions.透明质酸盐溶液中的构象与动态相互作用。
J Mol Biol. 1980 Apr;138(2):383-400. doi: 10.1016/0022-2836(80)90294-6.
5
Competitive inhibition evidence for specific intermolecular interactions in hyaluronate solutions.透明质酸盐溶液中特定分子间相互作用的竞争性抑制证据。
J Mol Biol. 1980 Apr;138(2):375-82. doi: 10.1016/0022-2836(80)90293-4.
6
Hyaluronic acid double helix.透明质酸双螺旋
J Mol Biol. 1983 Oct 5;169(4):861-72. doi: 10.1016/s0022-2836(83)80140-5.
7
Viscoelastic properties of hyaluronic acid and biological lubrication.
Univ Mich Med Cent J. 1968:255-9.
8
Self-association of hyaluronate segments in aqueous NaCl solution.
Arch Biochem Biophys. 1988 Sep;265(2):484-95. doi: 10.1016/0003-9861(88)90153-1.
9
Temporary network formation of hyaluronate under a physiological condition. 1. Molecular-weight dependence.生理条件下透明质酸的临时网络形成。1. 分子量依赖性。
Biopolymers. 1990;30(3-4):415-25. doi: 10.1002/bip.360300319.
10
Examination of corneal proteoglycans and glycosaminoglycans by rotary shadowing and electron microscopy.通过旋转阴影和电子显微镜检查角膜蛋白聚糖和糖胺聚糖。
Int J Biol Macromol. 1990 Jun;12(3):180-4. doi: 10.1016/0141-8130(90)90029-a.
Zotero 插件