Bailey Michelle, Correa Noemi, Harding Simon, Stone Nick, Brasselet Sophie, Palombo Francesca
School of Physics and Astronomy, University of Exeter, Exeter, EX4 4QL, UK.
Machine Intelligence Ltd, South Zeal, EX20 2JS, UK.
Data Brief. 2020 Feb 8;29:105267. doi: 10.1016/j.dib.2020.105267. eCollection 2020 Apr.
Brillouin spectroscopy, based on the inelastic scattering of light from thermally driven acoustic waves or [1], holds great promise in the field of life sciences as it provides functionally relevant micromechanical information in a contactless all-optical manner [2]. Due to the complexity of biological systems such as cells and tissues, which present spatio-temporal heterogeneities, interpretation of Brillouin spectra can be difficult. The data presented here were collected from gelatin hydrogels, used as tissue-mimicking model systems for Brillouin microspectroscopy measurements conducted using a lab-built Brillouin microscope with a dual-stage VIPA spectrometer. By varying the solute concentration in the range 4-18% (w/w), the macroscopic mechanical properties of the hydrogels can be tuned and the corresponding evolution in the Brillouin-derived longitudinal elastic modulus measured. An increase in Brillouin frequency shift with increasing solute concentration was observed, which was found to correlate with an increase in acoustic wave velocity and longitudinal modulus. The gels used here provide a viable model system for benchmarking and standardisation, and the data will be useful for spectrometer development and validation.
布里渊光谱基于光与热驱动声波的非弹性散射，在生命科学领域具有巨大潜力，因为它能以非接触式全光学方式提供功能相关的微机械信息。由于细胞和组织等生物系统的复杂性，存在时空异质性，布里渊光谱的解释可能具有挑战性。此处展示的数据是从明胶水凝胶收集而来，明胶水凝胶用作组织模拟模型系统，通过实验室搭建的配备双级可变干涉仪光谱仪的布里渊显微镜进行布里渊显微光谱测量。通过在4 - 18%（w/w）范围内改变溶质浓度，可以调节水凝胶的宏观力学性能，并测量布里渊衍生的纵向弹性模量的相应变化。观察到随着溶质浓度增加，布里渊频移增大，且发现这与声波速度和纵向模量的增加相关。此处使用的凝胶为基准测试和标准化提供了可行的模型系统，这些数据将有助于光谱仪的开发和验证。
