Vaughan J M, Randall J T
Nature. 1980 Apr 3;284(5755):489-91. doi: 10.1038/284489a0.
Brillouin spectra of biological systems may ultimately be related to their intrinsic molecular properties. In some instances the optical properties may be associated with the elastic ones and ultimately with the force constants of the molecules involved. In the present work we have used a triple-pass Fabry-Perot interferometer to measure Brillouin light scattering spectra for refractive tissues of the eye, including cornea, capsule and lens. Combined with corresponding measurements of density, estimates of the real and imaginary parts M' and M'' of the longitudinal bulk modulus have been made for the first time. Measurements have extended over four classes of vertebrate: Mammalia, Aves, Pisces and Amphibia; only small differences have been found between the various samples of cornea, whereas marked differences occur between the different lenses. Hence this account concentrates largely on the latter. The implications of this work lie not so much at the ophthalmological level as at the macromolecular and offer, in conjunction with other scattering techniques, opportunities for probing the lens and its proteins topographically as a function of growth.
生物系统的布里渊光谱最终可能与其内在分子特性相关。在某些情况下，光学特性可能与弹性特性相关，并最终与所涉及分子的力常数相关。在本研究中，我们使用了三通道法布里 - 珀罗干涉仪来测量眼睛屈光组织（包括角膜、晶状体囊和晶状体）的布里渊光散射光谱。结合密度的相应测量，首次对纵向体积模量的实部M'和虚部M''进行了估算。测量涵盖了四类脊椎动物：哺乳动物、鸟类、鱼类和两栖动物；角膜的各种样本之间仅发现了微小差异，而不同晶状体之间则存在显著差异。因此，本报告主要集中在后者。这项工作的意义与其说是在眼科层面，不如说是在大分子层面，并且与其他散射技术相结合，为从地形学角度探测晶状体及其蛋白质随生长的变化提供了机会。
