Department of Gastrointestinal Surgery, Shaoxing People's Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing, China.
Eur Rev Med Pharmacol Sci. 2020 Feb;24(3):1186-1194. doi: 10.26355/eurrev_202002_20170.
This study explored the effect of miR-26a-5p on cell proliferation, migration, and invasion in gastric cancer by targeting COL10A1.
First, differentially expressed genes were identified from microarray GSE103236 data of human gastric cancer. Then, qRT-PCR was carried out to detect the expression levels of COL10A1 and miR-26a-5p in gastric cancer cells and normal cases. The CCK-8 method was used to test cell proliferation. The colony formation assay was performed for the examination of the cell colony-forming ability, and transwell was applied for the detection of cell migration and invasion. Subsequently, the targeted relationship between miR-26a-5p and COL10A1 was identified by bioinformatics methods and further verified by Dual-Luciferase assay. The rescue experiment was finally conducted to validate the miR-26a-5p-dependent mechanism on cell proliferation, migration, and invasion via targeting COL10A1.
COL10A1 was found to be highly expressed in gastric cancer cells, while miR-26a-5p was poorly expressed. Silencing COL10A1 inhibited cell proliferation, migration, and invasion in gastric cancer. Besides, miR-26a-5p could function on gastric cancer cells by reducing COL10A1. As well, the rescue experiment suggested that the down-regulation of COL10A1 could reverse the inhibitory effect of miR-26a-5p on gastric cancer cells.
Collectively, miR-26a-5p can potentiate proliferation, migration, and invasion of gastric cancer cells by targeting COL10A1.
本研究通过靶向 COL10A1 探讨 miR-26a-5p 对胃癌细胞增殖、迁移和侵袭的影响。
首先，从人类胃癌 microarray GSE103236 数据中鉴定差异表达基因。然后，采用 qRT-PCR 检测胃癌细胞和正常病例中 COL10A1 和 miR-26a-5p 的表达水平。CCK-8 法检测细胞增殖。集落形成实验用于检测细胞集落形成能力，Transwell 用于检测细胞迁移和侵袭。随后，通过生物信息学方法鉴定 miR-26a-5p 和 COL10A1 之间的靶向关系，并通过双荧光素酶报告实验进一步验证。最后进行挽救实验，通过靶向 COL10A1 验证 miR-26a-5p 对细胞增殖、迁移和侵袭的依赖机制。
COL10A1 在胃癌细胞中高表达，而 miR-26a-5p 低表达。沉默 COL10A1 抑制胃癌细胞的增殖、迁移和侵袭。此外，miR-26a-5p 可以通过降低 COL10A1 来发挥作用。同样，挽救实验表明，下调 COL10A1 可以逆转 miR-26a-5p 对胃癌细胞的抑制作用。
综上所述，miR-26a-5p 通过靶向 COL10A1 增强胃癌细胞的增殖、迁移和侵袭。
