Department of Oncology, Jiangxi Maternal and Child Health Hospital, Nanchang, China.
Eur Rev Med Pharmacol Sci. 2020 Feb;24(3):1054-1061. doi: 10.26355/eurrev_202002_20155.
To elucidate the role of linc-UBC1 in regulating the metastasis and progression of ovarian cancer (OC) by downregulating the p53 level.
Relative levels of linc-UBC1 in OC tissues and paracancerous tissues were determined by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). Differential expressions of linc-UBC1 in OC tissues with different tumor staging or tumor sizes were detected as well. Receiver operating characteristic (ROC) curves were introduced for assessing the diagnostic value of linc-UBC1 in OC. After silence of linc-UBC1, proliferative and migratory abilities of HO8910 and HEY cells were evaluated. Subcellular distribution of linc-UBC1 was analyzed. The interaction between linc-UBC1 and p53 was explored through the RNA immunoprecipitation (RIP) assay. At last, rescue experiments were conducted to uncover the role of linc-UBC1/p53 regulatory loop in influencing the progression of OC.
Linc-UBC1 was upregulated in OC and its level negatively correlated to that of p53. Linc-UBC1 level was higher in OC patients with advanced TNM staging or larger tumor size. Linc-UBC1 was mainly distributed in the nucleus. Silence of linc-UBC1 attenuated proliferative and migratory abilities of HO8910 and HEY cells. RIP assay verified that linc-UBC1 could inhibit the transcription of p53. Knockdown of p53 could partially reverse the regulatory effects of linc-UBC1 on regulating the progression of OC.
Linc-UBC1 is upregulated in OC tissues and cells. It stimulates the proliferation and metastasis of OC by downregulating p53 level, thus exerting a carcinogenic role.
通过下调 p53 水平，阐明 linc-UBC1 在调节卵巢癌（OC）转移和进展中的作用。
通过实时定量聚合酶链反应（qRT-PCR）确定 OC 组织和癌旁组织中 linc-UBC1 的相对水平。检测 OC 组织中 linc-UBC1 的差异表达与不同肿瘤分期或肿瘤大小。引入受试者工作特征（ROC）曲线评估 linc-UBC1 在 OC 中的诊断价值。沉默 linc-UBC1 后，评估 HO8910 和 HEY 细胞的增殖和迁移能力。分析 linc-UBC1 的亚细胞分布。通过 RNA 免疫沉淀（RIP）实验探讨 linc-UBC1 和 p53 之间的相互作用。最后，进行挽救实验以揭示 linc-UBC1/p53 调节环在影响 OC 进展中的作用。
linc-UBC1 在 OC 中上调，其水平与 p53 的水平呈负相关。OC 患者中 linc-UBC1 水平在 TNM 分期较晚或肿瘤较大的患者中较高。linc-UBC1 主要分布在细胞核中。沉默 linc-UBC1 可减弱 HO8910 和 HEY 细胞的增殖和迁移能力。RIP 实验验证 linc-UBC1 可抑制 p53 的转录。下调 p53 可部分逆转 linc-UBC1 对调节 OC 进展的调节作用。
linc-UBC1 在 OC 组织和细胞中上调。它通过下调 p53 水平刺激 OC 的增殖和转移，从而发挥致癌作用。
