致癌多瘤病毒 BKPyV、JCPyV 和 SV40 的母婴垂直传播。

Mother-to-child transmission of oncogenic polyomaviruses BKPyV, JCPyV and SV40.

机构信息

Laboratories of Cell Biology and Molecular Genetics, Section of Pathology, Oncology and Experimental Biology, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 64/b, Fossato di Mortara Street, Ferrara 44121, Italy.

出版信息

J Infect. 2020 May;80(5):563-570. doi: 10.1016/j.jinf.2020.02.006. Epub 2020 Feb 22.

DOI:10.1016/j.jinf.2020.02.006

PMID:32097686

Abstract

OBJECTIVES

Polyomavirus (PyV) infections have been associated with different diseases. BK (BKPyV), JC (JCPyV) and simian virus 40 (SV40) are the three main PyVs whose primary infection occurs early in life. Their vertical transmission was investigated in this study.

METHODS

PyV sequences were analyzed by the digital droplet PCR in blood, serum, placenta, amniotic fluid, vaginal smear from two independent cohorts of pregnant females and umbilical cord blood (UCB) samples. IgG antibodies against the three PyVs were investigated by indirect E.L.I.S.As with viral mimotopes.

RESULTS

DNAs from blood, vaginal smear and placenta tested BKPyV-, JCPyV- and SV40-positive with a distinct prevalence, while amniotic fluids were all PyVs-negative. A prevalence of 3%, 7%, and 3% for BKPyV, JCPyV and SV40 DNA sequences, respectively, was obtained in UCBs. Serum IgG antibodies from pregnant females reached an overall prevalence of 62%, 42% and 17% for BKPyV, JCPyV and SV40, respectively. Sera from newborns (UCB) tested IgG-positive with a prevalence of 10% for BKPyV/JCPyV and 3% for SV40.

CONCLUSIONS

In this investigation, PyV vertical transmission was revealed by detecting PyV DNA sequences and IgG antibodies in samples from females and their offspring suggesting a potential risk of diseases in newborns.

摘要

目的

多瘤病毒（PyV）感染与多种疾病有关。BK（BKPyV）、JC（JCPyV）和猿猴病毒 40（SV40）是三种主要的 PyV，其原发性感染发生在生命早期。本研究调查了它们的垂直传播情况。

方法

通过数字液滴 PCR 分析了来自两个独立孕妇队列的血液、血清、胎盘、羊水、阴道涂片和脐带血（UCB）样本中的 PyV 序列。使用病毒模拟物通过间接 E.L.I.S.A 检测针对这三种 PyV 的 IgG 抗体。

结果

血液、阴道涂片和胎盘的 DNA 检测到 BKPyV、JCPyV 和 SV40 呈阳性，具有不同的流行率，而羊水均为 PyV 阴性。UCB 中分别获得 BKPyV、JCPyV 和 SV40 DNA 序列的流行率为 3%、7%和 3%。孕妇血清 IgG 抗体的总流行率分别为 BKPyV、JCPyV 和 SV40 的 62%、42%和 17%。新生儿（UCB）的血清 IgG 呈阳性，BKPyV/JCPyV 的流行率为 10%，SV40 的流行率为 3%。