Pathology Department, Farhet Hached University Hospital, 4000, Sousse, Tunisia.
Faculty of Sciences and Techniques of Sidi Bouzid, Kairouan University, Kairouan, Tunisia.
J Neurovirol. 2020 Jun;26(3):347-357. doi: 10.1007/s13365-020-00833-4. Epub 2020 Mar 2.
The gliomagenesis remains not fully established and their etiological factors still remain obscure. Polyomaviruses were detected and involved in several human tumors. Their potential implication in gliomas has been not yet surveyed in Africa and Arab World. Herein, we investigated the prevalence of six polyomaviruses (SV40, JCPyV, BKPyV, MCPyV, KIPyV, and WUPyV) in 112 gliomas from Tunisian patients. The DNA sequences of polyomaviruses were examined by PCR assays. Viral infection was confirmed by DNA in situ hybridization (ISH) and/or immunohistochemistry (IHC). The relationships between polyomavirus infection and tumor features were evaluated. Specific SV40 Tag, viral regulatory, and VP1 regions were identified in 12 GBM (10.7%). DNA ISH targeting the whole SV40 genome and SV40 Tag IHC confirmed the PCR findings. Five gliomas yielded JCPyV positivity by PCR and DNA ISH (2.7%). However, no BKPyV, KIPyV, and WUPyV DNA sequences were identified in all samples. MCPyV DNA was identified in 30 gliomas (26.8%). For GBM samples, MCPyV was significantly related to patient age (p = 0.037), tumor recurrence (p = 0.024), and SV40 (p = 0.045) infection. No further significant association was identified with the remaining tumor features (p > 0.05) and patient survival (Log Rank, p > 0.05). Our study indicates the presence of SV40, JCPyV, and MCPyV DNA in Tunisian gliomas. Further investigations are required to more elucidate the potential involvement of polyomaviruses in these destructive malignancies.
神经胶质瘤的发生机制尚未完全确立，其病因仍不清楚。多瘤病毒已被检测到并涉及多种人类肿瘤。它们在非洲和阿拉伯世界的胶质瘤中的潜在作用尚未得到调查。在此，我们研究了 112 例来自突尼斯患者的胶质瘤中六种多瘤病毒（SV40、JCPyV、BKPyV、MCPyV、KIPyV 和 WUPyV）的流行情况。通过 PCR 检测多瘤病毒的 DNA 序列。通过 DNA 原位杂交（ISH）和/或免疫组织化学（IHC）证实病毒感染。评估了多瘤病毒感染与肿瘤特征之间的关系。在 12 例 GBM（10.7%）中鉴定出特异性 SV40 Tag、病毒调节和 VP1 区。针对整个 SV40 基因组和 SV40 Tag IHC 的 DNA ISH 证实了 PCR 结果。5 例胶质瘤通过 PCR 和 DNA ISH 呈 JCPyV 阳性（2.7%）。然而，在所有样本中均未鉴定出 BKPyV、KIPyV 和 WUPyV DNA 序列。MCPyV DNA 在 30 例胶质瘤（26.8%）中被鉴定出。对于 GBM 样本，MCPyV 与患者年龄（p=0.037）、肿瘤复发（p=0.024）和 SV40 感染显著相关（p=0.045）。与其余肿瘤特征（p>0.05）和患者生存（Log Rank，p>0.05）无进一步显著相关性。我们的研究表明 SV40、JCPyV 和 MCPyV DNA 存在于突尼斯的胶质瘤中。需要进一步研究以更阐明多瘤病毒在这些破坏性恶性肿瘤中的潜在作用。
