Lellé R J, Heidenreich W, Borsi C, Stauch G, Gerdes J
Service de Gynécologie et Obstétrique, Université libre de Berlin.
J Gynecol Obstet Biol Reprod (Paris). 1988;17(4):485-9.
In 329 breast tissues (120 benign lesions and 209 invasive breast carcinomas) the growth fraction was determined using the monoclonal antibody Ki-67. The percentage of Ki-67 positive cells or the Ki-67 growth fraction was determined on histologic and cytologic specimens after immunoperoxidase staining. In benign breast lesions, the Ki-67 growth fraction never exceeded 11%, whereas in breast cancer 14.4 +/- 9.9% of cells were Ki-67 positive ranging from 1 to 48%. Breast carcinomas were classified according to the histologic grading system of Bloom and Richardson (G1-G3). Poorly differentiated carcinomas. (G3) had significantly higher Ki-67 growth fractions than well differentiated tumors (G1). Ki-67 was also correlated with axillary lymph node status. Growth fractions of N+ carcinomas were significantly higher than those of N0 tumors (16.4 +/- 10.4% versus 12.4 +/- 9.4%; p = 0.006). Thus, this new method yields similar results to those obtained by other researchers through the use of flow cytometry and thymidine labelling. As determination of Ki-67 growth fractions is an easy and rapid technique, it is ideal for routine clinical use. Ki-67 may be useful in prognosis and in the selection of patients for various treatment modalities such as adjuvant therapy. A wide-spread use of Ki-67 would be helpful to gain further experience.
在329份乳腺组织样本（120份良性病变样本和209份浸润性乳腺癌样本）中，使用单克隆抗体Ki-67测定生长分数。免疫过氧化物酶染色后，在组织学和细胞学标本上测定Ki-67阳性细胞的百分比或Ki-67生长分数。在良性乳腺病变中，Ki-67生长分数从未超过11%，而在乳腺癌中，14.4±9.9%的细胞Ki-67呈阳性，范围为1%至48%。乳腺癌根据Bloom和Richardson的组织学分级系统（G1-G3）进行分类。低分化癌（G3）的Ki-67生长分数显著高于高分化肿瘤（G1）。Ki-67也与腋窝淋巴结状态相关。N+癌的生长分数显著高于N0肿瘤（分别为16.4±10.4%和12.4±9.4%；p = 0.006）。因此，这种新方法产生的结果与其他研究人员通过流式细胞术和胸腺嘧啶核苷标记法获得的结果相似。由于测定Ki-67生长分数是一种简单快速的技术，非常适合常规临床应用。Ki-67在预后评估以及为辅助治疗等各种治疗方式选择患者方面可能有用。广泛使用Ki-67将有助于积累更多经验。
