Kalleas Dimitrios, McEvoy Keith, Horne Gregory, Roberts Stephen A, Brison Daniel R
Department of Reproductive Medicine, Old St Mary's Hospital Manchester University NHS Foundation Trust, Manchester Academic Health Sciences Centre, Manchester, UK.
Centre for Biostatistics, Division of Population Health, Health Services Research & Primary Care, School of Health Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.
Hum Fertil (Camb). 2022 Feb;25(1):147-153. doi: 10.1080/14647273.2020.1729423. Epub 2020 Feb 26.
Time-lapse (TL) incubators are increasingly used in fertilization (IVF) laboratories but there have been few studies of their effectiveness in comparison to other incubator types. Moreover, the design of most studies has been limited by the quality of the control incubator. We have therefore performed a one-year pseudo-randomized prospective study of IVF cycles using a TL incubator (EmbryoScope™) ( = 243) or a conventional incubator (K-System G-185 Flatbed) ( = 203). The two groups were well matched in terms of clinical parameters: IVF cycle attempt number, IVF/ICSI, age, number and day (3 or 5) of embryo transfer. Embryos were selected for transfer using conventional (non-TL) morphological grading. The EmbryoScope group had an increased chance of a live birth (43.2% vs. 34.5%; OR = 1.43 [95%CI: 0.96-2.13]) with significantly reduced early pregnancy loss (5.8% vs. 13.8%; OR = 0.37 [0.19-0.74]) compared to the K-System incubator. There was a higher proportion of 4-cell embryos on day 2 and 8-cell embryos on day 3 in the EmbryoScope, compared to the K-Systems. The use of TL incubators is appropriate in ART by virtue of their high specification, facility for low oxygen culture and provision of minimally disturbed culture conditions which limit exposure of human embryos to environmental stress.
延时（TL）培养箱在体外受精（IVF）实验室中的使用越来越广泛，但与其他类型的培养箱相比，对其有效性的研究却很少。此外，大多数研究的设计都受到对照培养箱质量的限制。因此，我们进行了一项为期一年的伪随机前瞻性研究，比较使用TL培养箱（EmbryoScope™）（n = 243）或传统培养箱（K-System G-185平板）（n = 203）的IVF周期。两组在临床参数方面匹配良好：IVF周期尝试次数、IVF/ICSI、年龄、胚胎移植数量和日期（第3天或第5天）。使用传统（非TL）形态学分级选择胚胎进行移植。与K-System培养箱相比，EmbryoScope组活产几率增加（43.2%对34.5%；OR = 1.43 [95%CI：0.96 - 2.13]），早期妊娠丢失显著减少（5.8%对13.8%；OR = 0.37 [0.19 - 0.74]）。与K-System相比，EmbryoScope中第2天4细胞胚胎和第3天8细胞胚胎的比例更高。由于TL培养箱具有高规格、低氧培养设施以及提供最小干扰的培养条件，从而限制了人类胚胎暴露于环境压力，因此在辅助生殖技术中使用TL培养箱是合适的。
