Area for Molecular Function, Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan; Medical Innovation Research Unit (MiU), Advanced Institute of Innovative Technology (AIIT), Saitama University, Sakura, Saitama 338-8570, Japan.
Area for Molecular Function, Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan.
Bioorg Med Chem Lett. 2020 Apr 15;30(8):127024. doi: 10.1016/j.bmcl.2020.127024. Epub 2020 Feb 19.
A convenient assembly of fluorogenic glycopolymers having various polymer compositions was accomplished from the corresponding glycomonomer and dansyl monomer by means of radical polymerization, and the water-soluble glycopolymers gave typical fluorescence spectroscopic profiles due to the dansyl moieties on the glycopolymer in aqueous media. Biological evaluation of the polymer against wheat germ agglutinin (WGA) was accomplished on the basis of fluorescence changes due to tryptophan residues on WGA, and the affinities between the glycopolymers and WGA were estimated to be 4.7 × 10 to 9.3 × 10 M. In order to apply the fluorogenic glycopolymers for further biological measurements, efficient resonance energy transfer from tryptophan moieties on WGA to dansyl moieties on the fluorogenic glycopolymers was examined. FRET profiles of both fluorophores were similar compared to the binding profiles on the basis of fluorescence changes of tryptophan residues. This approach is applicable for the determination of an affinity constant between a carbohydrate and a lectin in which no fluorophore exists near the binding site.
通过自由基聚合，将相应的糖单体和丹磺酰单体方便地组装成具有各种聚合物组成的荧光糖聚合物，并且由于在水介质中的糖聚合物上的丹磺酰部分，水溶性糖聚合物给出了典型的荧光光谱特征。基于在 WGA 上色氨酸残基引起的荧光变化，对聚合物与麦胚凝集素（WGA）的生物评价进行了评估，并且估计糖聚合物和 WGA 之间的亲和力为 4.7×10 至 9.3×10 M。为了将荧光糖聚合物进一步应用于生物测量，检查了 WGA 上的色氨酸残基与荧光糖聚合物上的丹磺酰基之间的有效共振能量转移。与基于色氨酸残基荧光变化的结合曲线相比，两种荧光团的 FRET 曲线相似。该方法适用于确定碳水化合物与不存在于结合部位附近的荧光团的凝集素之间的亲和常数。
