Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA and Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Columbus, OH, USA.
Int J Nanomedicine. 2020 Feb 3;15:761-777. doi: 10.2147/IJN.S238445. eCollection 2020.
Salmonellosis in poultry is a serious economic burden. A major concern is the public health hazard caused by consumption of contaminated poultry products. Currently used vaccines are ineffective in combating poultry Salmonellosis warranting the need of a potent vaccine, especially an oral vaccine that can elicit robust local intestinal immunity.
A subunit chitosan nanoparticles (NPs)-based vaccine was prepared that contained immunogenic outer membrane proteins (OMPs) and -flagellin (F) protein (OMPs-F-CS NPs). OMPs-F-CS NPs were administered as an oral vaccine in layer chickens and the resultant humoral and cell-mediated immune responses and localization of NPs were examined using standard detection methods.
We demonstrated targeting of surface F-protein coated chitosan NPs to immune cells when delivered orally to layer chickens, the particles were localized in ileal Peyer's patches. The OMPs-F-CS NPs vaccinated layer chickens had significantly higher OMPs-specific mucosal IgA production and lymphocyte proliferation response. The candidate vaccine increased the expression of toll-like receptor (TLR)-2, TLR-4, IFN-γ, TGF-ß and IL-4 mRNA expression in chicken cecal tonsils.
Our study demonstrated that the chitosan-based oral nanovaccine targets immune cells of chickens and induced antigen-specific B and T cell responses. This candidate oral nanovaccine has the potential to mitigate Salmonellosis in poultry.
禽沙门氏菌病是一种严重的经济负担。人们主要关注的是食用受污染的家禽产品对公众健康造成的危害。目前使用的疫苗在防治禽沙门氏菌病方面效果不佳，因此需要一种有效的疫苗，特别是一种能够引起强烈局部肠道免疫的口服疫苗。
制备了一种基于壳聚糖纳米粒子（NPs）的疫苗，其中包含免疫原性的外膜蛋白（OMPs）和-鞭毛蛋白（F）蛋白（OMPs-F-CS NPs）。将 OMPs-F-CS NPs 作为口服疫苗施用于蛋鸡，使用标准检测方法检测由此产生的体液和细胞介导的免疫反应以及 NPs 的定位。
我们证明了口服给予蛋鸡时，表面 F 蛋白包被壳聚糖 NPs 可靶向免疫细胞，这些颗粒定位于回肠派尔集合淋巴结。用 OMPs-F-CS NPs 免疫的蛋鸡产生了明显更高水平的 OMPs 特异性黏膜 IgA 产生和淋巴细胞增殖反应。候选疫苗增加了鸡盲肠扁桃体中 TLR-2、TLR-4、IFN-γ、TGF-β和 IL-4 mRNA 表达。
我们的研究表明，壳聚糖基口服纳米疫苗可靶向鸡的免疫细胞，并诱导抗原特异性 B 和 T 细胞反应。这种口服纳米候选疫苗有可能减轻家禽的沙门氏菌病。
