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表没食子儿茶素 3 - O - 没食子酸酯对利什曼原虫的抗虫及免疫调节作用：细胞凋亡与基因表达谱分析

Anti-Leishmanial and Immunomodulatory Effects of Epigallocatechin 3-O-Gallate on : Apoptosis and Gene Expression Profiling.

作者信息

Saduqi Morteza, Sharifi Iraj, Babaei Zahra, Keyhani Alireza, Mostafavi Mahshid, Hakimi Parizi Maryam, Ghasemian Mahdiyeh, Bamorovat Mehdi, Sharifi Fatemeh, Aflatoonian Mohammad Reza, Sharififar Fariba, Ghasemi Nejad Pooya, Khosravi Ahmad, Salarkia Ehsan, Varma Rajender S

机构信息

Department of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.

Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

出版信息

Iran J Parasitol. 2019 Oct-Dec;14(4):521-533.

PMID:32099555
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7028242/
Abstract

BACKGROUND

Pentavalent antimonials such as meglumine antimoniate (MA, Glucantime), are the first-line treatment against leishmaniasis, but at present, they have basically lost their efficacy. This study was aimed to explore epigallocatechin 3-O-gallate (EGCG), alone or in combination with MA against stages.

METHODS

All experiments were carried out in triplicate using colorimetric assay, macrophage model, flow cytometry and quantitative real-time PCR. This experimental study was carried out in 2017 in Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

RESULTS

Promastigotes and amastigotes were more susceptible to EGCG than MA alone, but the effect was more profound when used in combination. EGCG exhibited high antioxidant level with a remarkable potential to induce apoptosis. Furthermore, the results showed that the level of gene expression pertaining to Th-1 was significantly up-regulated (<0.001).

CONCLUSION

EGCG demonstrated a potent anti-leishmanial effect alone and more enhanced lethal activity in combination. The principal mode of action entails the stimulation of a synergistic response and up-regulation of the immunomodulatory role towards Th-1 response against

摘要

背景

五价锑化合物如葡甲胺锑酸盐（MA，葡糖胺锑）是治疗利什曼病的一线药物，但目前它们基本已失去疗效。本研究旨在探究表没食子儿茶素3 - O - 没食子酸酯（EGCG）单独或与MA联合对抗各阶段（利什曼病）的效果。

方法

所有实验均一式三份进行，采用比色法、巨噬细胞模型、流式细胞术和定量实时聚合酶链反应。本实验研究于2017年在伊朗克尔曼医科大学利什曼病研究中心开展。

结果

前鞭毛体和无鞭毛体对EGCG的敏感性高于单独使用MA，但联合使用时效果更显著。EGCG表现出高抗氧化水平，具有显著的诱导凋亡潜力。此外，结果表明与Th - 1相关的基因表达水平显著上调（<0.001）。

结论

EGCG单独显示出强大的抗利什曼原虫作用，联合使用时致死活性更强。主要作用方式包括刺激协同反应以及上调针对……的Th - 1反应的免疫调节作用

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/4f77a1bb0dba/IJPA-14-521-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/a56c566e728a/IJPA-14-521-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/88d6822bcd2a/IJPA-14-521-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/072c5db8115a/IJPA-14-521-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/236c68c968b7/IJPA-14-521-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/7ae2d091e38f/IJPA-14-521-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/dd0f3311bc1c/IJPA-14-521-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/4f77a1bb0dba/IJPA-14-521-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/a56c566e728a/IJPA-14-521-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/88d6822bcd2a/IJPA-14-521-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/072c5db8115a/IJPA-14-521-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/236c68c968b7/IJPA-14-521-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/7ae2d091e38f/IJPA-14-521-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/dd0f3311bc1c/IJPA-14-521-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a78/7028242/4f77a1bb0dba/IJPA-14-521-g007.jpg

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