The potential effect of silver nanoparticles synthesized with green seeds on proliferation, cytotoxicity activity, and cytokines expression level.

The goal of this study was to analyze the antileishmanial and antibacterial activity of green seed biosynthesize silver nanoparticles ( AgNPs), as well as cytotoxicity and cytokine gene expression. UV-vis spectroscopy, FTIR, and FESEM methods used to examine the AgNPs. MTT test was used to assess the antileishmanial and cytotoxicity effects. The gene expression level was assessed in NPs-treated J774 cells by qPCR. The synthesized AgNPs were in the size range of 20-70 nm, through FESEM pictures. The IC values of the NPs were 65. 4 and 47.70 μg/mL against promastigotes and amastigotes of , but these values were 580.1 and 171.1 μg/mL for Glucantime® as the control drug. AgNPs represented a significant increase in IL-12P40, as a Th1 cytokine, in comparison to Glucantime® at high concentrations ( < 0.01), whilst IL-10 expression level showed a significant reduction between NPs-treated and Glucantime®-treated macrophages at 250-1000 μg/mL concentrations ( < 0.001). Moreover, the NPs were cytotoxic on cancer cell lines of Hek293, MCF7, and A172 with the CC values of 437.2, 116.8, and 72.9 µg/mL, respectively. It showed a significant effect of these NPs against A172 ( < 0.001). Also, the lowest MIC values of the NPs were obtained for and (204 µg/mL). According to the antileishmanial, anticancer, and antibacterial activity of these NPs, it can considered a bio-agent drug in the future in endemic countries.