A Metabolomic Investigation of the Effect of Eosin B on Game-tocyte of 1HNMR Spectroscopy.

BACKGROUND

Recently eosin B was shown to have an effect on the asexual stage of and in this study, its activity against gametocytes and changes in the culture medium metabolites were investigated using anHNMR-based metabolomics approach.

METHODS

In the Biochemistry Department of Pasteur Institute of Iran in 2017, parasites were cultured and gametocytogenesis induced by heparin and 5% hematocrit. Sexual stage parasites were tested by eosin B in 90 well plates and IC determined using Lactate Dehydrogenase assay. Gametocytes were treated by IC dose of eosin B and the medium collected in the two groups: with eosin B and controls and sent for HNMR spectroscopy. The spectra were analyzed on MATLAB interface and the altered metabolites in the culture medium and eosin-affected biochemical pathways were identified by Human Metabolome Database and Metabo-analyst website.

RESULTS

The results revealed eosin B had an effective gametocytocidal activity against . The significant metabolites changed in the medium were thia-mine, Asp, Asn, Tyr, Lys, Ala, Phenylpyruvic acid, NAD and lipids. The main pathways identified were aminoacyl-tRNA biosynthesis, Phenylalanine, tyrosine and tryptophan biosynthesis, Alanine, aspartate and glutamate metabolism, Phenylala-nine metabolism, Nicotinate and nicotinamide metabolism, and lysine degradation.

CONCLUSION

Eosin B exhibited substantial gametocytocidal activity and affected important drug targets in the .