从伊朗北部绵羊中分离出的苯并咪唑抗性的分子研究。
Molecular Study of Benzimidazole Resistance in Isolated from Sheep in North of Iran.
作者信息
Nemati Rahim, Bahari Aliasghar, Mahmoodi Pezhman, Sazmand Alireza
机构信息
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
Department of Clinical Sciences, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
出版信息
Iran J Parasitol. 2019 Oct-Dec;14(4):646-651.
BACKGROUND
Resistance to benzimidazole (BZ) compounds is common in populations in sheep and goats worldwide. Given the importance of anthelmintic resistance and shortage of information on single nucleotide polymorphisms (SNPs) in this prevalent nematode in Iran, this study was conducted.
METHODS
From June to September 2016, abomasa of 139 sheep of different sexes and ages in Amol City slaughterhouse, northern Iran were examined for isolation of nematodes. Totally 45 male confirmed by both microscopical and nested-PCR-RFLP methods were included in this study. Susceptibility or resistance of each single worm to benzimidazoles was assessed using allele-specific PCR.
RESULTS
Frequency of genotypes in the present study were 33.33% heterozygote BZ and 66.67% BZ homozygote sensitive. No homozygote resistant worm was found.
CONCLUSION
Resistance against BZs in of sheep has occurred at a low prevalence in the north of Iran. However, mutated genes might get dominant under drug selection in future. Hence, periodic investigations for early detection of mutated alleles in nematode populations using accurate and sensitive molecular methods such as PCR-RFLP is recommended.
背景
在全球绵羊和山羊群体中，对苯并咪唑（BZ）类化合物产生抗性的情况很常见。鉴于抗蠕虫药抗性的重要性以及伊朗这种常见线虫中单核苷酸多态性（SNP）信息的匮乏，开展了本研究。
方法
2016年6月至9月，对伊朗北部阿莫勒市屠宰场的139只不同性别和年龄的绵羊的皱胃进行检查，以分离线虫。本研究共纳入了45只经显微镜检查和巢式PCR-RFLP方法确认的雄性线虫。使用等位基因特异性PCR评估每只线虫对苯并咪唑的敏感性或抗性。
结果
本研究中基因型的频率为33.33%杂合子BZ和66.67%BZ纯合子敏感型。未发现纯合子抗性线虫。
结论
伊朗北部绵羊中对BZ类药物的抗性以低流行率出现。然而，突变基因在未来的药物选择下可能会占主导地位。因此，建议定期使用准确且灵敏的分子方法如PCR-RFLP对线虫群体中的突变等位基因进行早期检测。
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