Opasawatchai Anunya, Yolwong Watchareewan, Thuncharoen Walairat, Inrueangsri Nanthicha, Itsaradisaikul Sulak, Sasisakulporn Cherapat, Jotikasthira Wanlapa, Matangkasombut Oranart, Reamtong Onrapak, Manuyakorn Wiparat, Songnuan Wisuwat, Matangkasombut Ponpan
Department of Microbiology, Faculty of Science, Mahidol University, Thailand.
Faculty of Dentistry, Mahidol University, Thailand.
World Allergy Organ J. 2020 Feb 17;13(2):100099. doi: 10.1016/j.waojou.2020.100099. eCollection 2020 Feb.
Mosquito allergy is common in tropical countries but remains under-diagnosed. This may be due to the lack of knowledge and diagnostic tools for tropical mosquito allergens.
We aimed to characterize allergens from tropical mosquito species and investigate IgE reactivity in mosquito-allergic patients to the salivary gland proteins from these mosquitoes.
Salivary gland extract (SGE) from 4 mosquito species, highly distributed in the tropics, including and were studied. SGE-specific IgE and IgG ELISA were developed, and serum from 64 mosquito-allergic and 22 non-allergic healthy control subjects was assayed. Further investigations using IgE-immunoblots followed by mass spectrometry analysis were performed to identify and characterize allergens from each species.
Mosquito-allergic subjects have detectable serum IgE to SGE derived from local mosquito species, while the IgE levels to using commercially available ELISA were mostly minimal. IgE-immunoblot analysis and mass spectrometry identified 5 novel mosquito allergens from (Aed al 2, Aed al 3), (Cul q 2.01, Cul q 3), and (Ano d 2). Interestingly, 4 of the 5 new allergens belong to the D7 protein family.
CONCLUSIONS & CLINICAL RELEVANCE: Five novel allergens from 3 tropical mosquito species were characterized. The majority of mosquito-allergic subjects who live in the tropics have IgE reactivity to these allergens. Our study paves the way for the development of diagnostic tests, component-resolved diagnostics, and future immunotherapy for mosquito allergy in tropical countries.
蚊虫过敏在热带国家很常见，但仍未得到充分诊断。这可能是由于缺乏对热带蚊虫过敏原的了解和诊断工具。
我们旨在鉴定来自热带蚊虫种类的过敏原，并研究蚊虫过敏患者对这些蚊虫唾液腺蛋白的IgE反应性。
研究了4种在热带地区广泛分布的蚊虫的唾液腺提取物（SGE），包括[具体蚊虫种类1]、[具体蚊虫种类2]、[具体蚊虫种类3]和[具体蚊虫种类4]。开发了SGE特异性IgE和IgG ELISA，并检测了64名蚊虫过敏患者和22名非过敏健康对照受试者的血清。进一步采用IgE免疫印迹法，随后进行质谱分析，以鉴定和表征每种蚊虫的过敏原。
蚊虫过敏受试者对来自当地蚊虫种类的SGE可检测到血清IgE，而使用市售ELISA检测对[具体蚊虫种类]的IgE水平大多极低。IgE免疫印迹分析和质谱鉴定出5种来自[具体蚊虫种类1]（埃及伊蚊2、埃及伊蚊3）、[具体蚊虫种类2]（致倦库蚊2.01、致倦库蚊3）和[具体蚊虫种类3]（白纹伊蚊2）的新型蚊虫过敏原。有趣的是，5种新过敏原中有4种属于D7蛋白家族。
鉴定出了3种热带蚊虫种类的5种新型过敏原。大多数生活在热带地区的蚊虫过敏受试者对这些过敏原具有IgE反应性。我们的研究为热带国家蚊虫过敏的诊断测试、组分分辨诊断及未来免疫治疗的发展铺平了道路。
