Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xinning road, Xining 810001, P. R. China.
Institute of Light Industry in Qinghai Province, 2 Qingnian road, Xining 810008, P.R. China.
J Chromatogr Sci. 2020 Apr 22;58(3):211-216. doi: 10.1093/chromsci/bmz091.
Oxytropis falcata Bunge, known as "the King of Herbs" in Tibetan medicine, is used for treatment of hyperpyrexia, pain, wounds, inflammation and anthrax. However, it is difficult to isolate compound with high-purity from O. falcata because of its complexity. In this work, an efficient method was successfully established for the separation of 3-hydroxy-3-methylglutaryl (HMG) flavonoid glycosides from O. falcata by 2D preparative chromatography (2D-HPLC). An ODS C18 preparative column was used for the first-dimension preparation, and the XCharge C18 preparative column with different separation selectivity to the ODS C18 stationary phase was used for the second-dimension preparation. Four HMG flavonoid glycosides (oxytroflavosides A-D) and two flavonoid glycosides (oxytroflavosides F and G) were separated with purities over 98%. Their structures were elucidated by nuclear magnetic resonance spectroscopy. The 2D-HPLC method used in this study is effective for preparative separation of HMG flavonoid glycosides from O. falcata. Additionally, this method shows great potential for the separation of flavonoid glycosides from other plant extracts.
藏药中的“草中王”——麻花艽，具有解热、镇痛、消炎、炭疽等功效。但其成分复杂，从麻花艽中分离高纯度的化合物比较困难。本工作采用二维制备色谱（2D-HPLC）成功建立了从麻花艽中分离 3-羟基-3-甲基戊二酰基（HMG）黄酮苷的有效方法。采用 ODS C18 制备柱进行一维制备，采用对 ODS C18 固定相具有不同分离选择性的 XCharge C18 制备柱进行二维制备。分离得到的四个 HMG 黄酮苷（oxytroflavosides A-D）和两个黄酮苷（oxytroflavosides F 和 G）的纯度均大于 98%。通过核磁共振波谱法阐明了它们的结构。本研究中使用的 2D-HPLC 方法可有效用于从麻花艽中制备分离 HMG 黄酮苷。此外，该方法有望用于从其他植物提取物中分离黄酮苷。
