Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.
Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining, P. R. China.
J Sep Sci. 2020 Nov;43(21):4018-4027. doi: 10.1002/jssc.202000688. Epub 2020 Oct 13.
Flavonoid glycosides exist widely in medicine herbs and often used as nutraceuticals because of their excellent bioactivity and low toxicity. For accurate quality control and bioactivity assessment of Sphaerophysa salsula, a rapid and productive method to isolate flavonoid glycosides is needed. Therefore, this work reports the development of a novel comprehensive strategy based on an online middle-pressure chromatography and preparative high-performance liquid chromatography for rapid enrichment and separation of flavonoid glycosides from S. salsula. First, the flavonoid glycosides were enriched using an online middle-pressure chromatographic column containing stationary middle chromatogram isolated phase. During this process, the high-volume injection of the extracting solution was realized by an empty precolumn positioned before the main chromatographic tower. Then, the compounds were separated through preparative high-performance liquid chromatography with Megress C18. As a result, one new flavonol 3-O-glycoside (2) and two known flavonol 3-O-glycosides (1, 3) were targetedly isolated from S. salsula. The content of compounds 1-3 in S. salsula was 0.09, 0.11, and 0.18 wt%, respectively. Comparing to traditional enrichment and separation methods, our technique offers significantly shorter sample pretreatment time as well as high reproducibility. We believe that our separation method has a strong potential to be used for the processing of other medicinal plants.
类黄酮糖苷广泛存在于草药中，由于其出色的生物活性和低毒性，常被用作营养保健品。为了准确控制沙拐枣的质量并评估其生物活性，需要开发一种快速、高效的方法来分离类黄酮糖苷。因此，本工作报道了一种基于在线中压色谱和制备高效液相色谱的新型综合策略，用于快速富集和分离沙拐枣中的类黄酮糖苷。首先，使用含有固定相中压色谱柱对类黄酮糖苷进行富集，在此过程中，通过位于主色谱塔前的空前置柱实现提取溶液的大体积进样。然后，通过 Megress C18 制备高效液相色谱对化合物进行分离。结果，从沙拐枣中靶向分离得到一个新的黄酮醇 3-O-糖苷（2）和两个已知的黄酮醇 3-O-糖苷（1、3）。化合物 1-3 在沙拐枣中的含量分别为 0.09、0.11 和 0.18wt%。与传统的富集和分离方法相比，我们的技术提供了更短的样品预处理时间和更高的重现性。我们相信，我们的分离方法具有很强的潜力，可用于其他药用植物的加工。
