嗜酸铁硫杆菌在淡水和盐水环境中对黄铁矿的附着以及对 Langmuir 和 Freundlich 等温线的拟合。
Attachment of Acidithiobacillus ferrooxidans to pyrite in fresh and saline water and fitting to Langmuir and Freundlich isotherms.
机构信息
Department of Metallurgical Engineering and Materials, Universidad Técnica Federico Santa María, Vicuña Mackenna 3939, Santiago, Chile.
Department of Mining Engineering, Universidad de Chile, Beauchef 850, Santiago, Chile.
出版信息
Biotechnol Lett. 2020 Jun;42(6):957-964. doi: 10.1007/s10529-020-02842-z. Epub 2020 Feb 25.
OBJECTIVE
This study aims to investigate the attachment of Acidithiobacillus ferrooxidans to pyrite in two different environments: fresh and saline water (water with 35 g/L of NaCl or 0.6 M). Adsorption isotherms were analyzed using the Langmuir and Freundlich models. Saline water is water with 35 g/L of NaCl (0.6 M), which is the concentration of NaCl in seawater. The use of raw seawater in mining is becoming relevant in leaching and flotation process. At the same time the use of microorganisms in both processes is gaining attention. For this reason, it is important to study the behavior of adherence of microorganisms to minerals in saline aqueous environments, similar to seawater.
RESULTS
The bacteria showed a higher level of attachment to pyrite in fresh water than in saline water. The Langmuir model fitted better the experimental data obtained in fresh water than in saline water with a coefficient of determination (R) of 0.85 and 0.61 for fresh and saline water, respectively.
CONCLUSIONS
This suggests that the bacteria tend to adhere more as a monolayer in fresh than in saline water in the early stage of adhesion.
目的
本研究旨在探究嗜酸氧化亚铁硫杆菌在新鲜水和盐水（含 35 g/L NaCl 或 0.6 M）两种不同环境下对黄铁矿的附着情况。采用 Langmuir 和 Freundlich 模型对吸附等温线进行分析。盐水是指含有 35 g/L NaCl（0.6 M）的水，即海水中 NaCl 的浓度。在浸出和浮选过程中，使用原海水进行采矿变得越来越重要。同时，微生物在这两个过程中的应用也受到了关注。因此，研究微生物在类似海水的盐水中对矿物的附着行为非常重要。
结果
与在盐水中相比，细菌在新鲜水中对黄铁矿的附着程度更高。Langmuir 模型更适合于在新鲜水中获得的实验数据，其决定系数（R）为 0.85，而在盐水中的 R 为 0.61。
结论
这表明在附着的早期阶段，细菌在新鲜水中比在盐水中更倾向于单层附着。
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