Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, Hunan, China,
Biotechnol Lett. 2013 Dec;35(12):2129-36. doi: 10.1007/s10529-013-1316-1. Epub 2013 Aug 24.
Attachments of Acidithiobacillus ferrooxidans ATCC 23270 onto elemental sulfur, quartz and complex chalcopyrite were investigated by analysis of its extracellular polymeric substances as well as applying Langmuir and Freundlich equations. The two equations fitted the adsorption equilibrium data with significant correlation coefficient over 0.9. This indicated that bacterial attachment is complicated and involves Langmuir and Freundlich characterizations. Sulfur-grown cells showed the highest affinity for the three solid substrates. The investigated complex chalcopyrite possessed a higher maximum adsorption capacity for A. ferrooxidans than elemental sulfur or quartz. The Freundlich fitting parameters suggested that quartz had a weaker adsorption capacity and smaller adsorption areas than elemental sulfur or the complex chalcopyrite. It is not the content of total carbohydrates or proteins in EPS but their ratios that determine the affinity differences between cells and substrates.
通过分析嗜酸氧化亚铁硫杆菌 ATCC 23270 的胞外聚合物以及应用朗缪尔和弗伦德利希方程，研究了其对元素硫、石英和复杂黄铜矿的附着情况。这两个方程用相关系数大于 0.9 的显著性拟合了吸附平衡数据。这表明细菌附着是复杂的，涉及朗缪尔和弗伦德利希特征。硫磺生长的细胞对三种固体基质表现出最高的亲和力。所研究的复杂黄铜矿对嗜酸氧化亚铁硫杆菌的最大吸附容量高于元素硫或石英。弗伦德利希拟合参数表明，石英的吸附能力比元素硫或复杂黄铜矿弱，吸附面积也更小。决定细胞与基质之间亲和力差异的不是 EPS 中总碳水化合物或蛋白质的含量，而是它们的比例。
