三叶状瓣片组织构建的组织工程。
tissue engineering of a trilayered leaflet-shaped tissue construct.
机构信息
Department of Bioengineering, University of Missouri, Columbia, MO 65211, USA.
Division of Cardiovascular Diseases, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
出版信息
Regen Med. 2020 Jan;15(1):1177-1192. doi: 10.2217/rme-2019-0078. Epub 2020 Feb 26.
Abstract
We aimed to develop a leaflet-shaped trilayered tissue construct mimicking the morphology of native heart valve leaflets. Electrospinning and tissue engineering methods were employed. We developed leaflet-shaped microfibrous scaffolds, each with circumferentially, randomly and radially oriented three layers mimicking the trilayered, oriented structure of native leaflets. After 3 months tissue engineering with the scaffolds, the generated leaflet-shaped tissue constructs had a trilayered structure mimicking the orientations of native heart valve leaflets. Presence of collagen, glycosaminoglycans and elastin seen in native leaflets was observed in the engineered tissue constructs. Trilayered, oriented fibrous scaffolds brought the orientations of the infiltrated cells and their produced extracellular matrix proteins into the constructs.
摘要
我们旨在开发一种模仿天然心脏瓣膜叶形态的叶片状三层组织构建体。采用静电纺丝和组织工程方法。我们开发了叶片状微纤维支架，每个支架都具有周向、随机和放射状取向的三层，模仿天然叶瓣的三层定向结构。经过 3 个月的支架组织工程化，生成的叶片状组织构建体具有模仿天然心脏瓣膜叶取向的三层结构。在工程组织构建体中观察到了在天然叶瓣中存在的胶原、糖胺聚糖和弹性蛋白。三层定向纤维支架将渗透细胞的取向及其产生的细胞外基质蛋白带入构建体中。
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