Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, 7610000, Israel.
The Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, 7610000, Israel.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1656-1665. doi: 10.1099/ijsem.0.003952.
The genus (Family: ; Order: Class: Alphaproteobacteria) comprises facultative intracellular Gram-negative, haemotropic, slow-growing, vector-borne bacteria. Wild rodents and their fleas harbor a great diversity of species and strains of the genus , including several zoonotic ones. This genetic diversity coupled with a fastidious nature of the organism results in a taxonomic challenge that has led to a massive collection of uncharacterized strains. Here, we report the genomic and phenotypic characterization of two strains, members of the genus (namely Tel Aviv and OE 1-1), isolated from rats and fleas, respectively. Scanning electron microscopy revealed rod-shaped bacteria with polar pili, lengths ranging from 1.0 to 2.0 µm and widths ranging from 0.3 to 0.6 µm. OE 1-1 and Tel Aviv strains contained one single chromosome of 2.16 and 2.23 Mbp and one plasmid of 29.0 and 41.5 Kbp, with average DNA G+C contents of 38.16 and 38.47 mol%, respectively. These strains presented an average nucleotide identity (ANI) of 89.9 %. was found to be the closest phylogenetic relative of both strains (ANI=90.9-93.6 %). The major fatty acids identified in both strains were Cω7, C and C. They differ from in their C and C compositions. Both strains are strictly capnophilic and their biochemical profiles resembled those of species of the genus with validly published names, whereas differences in arylamidase activities partially assisted in their speciation. Genomic and phenotypic differences demonstrate that OE 1-1 and Tel Aviv strains represent novel individual species, closely related to , for which we propose the names sp. nov. and sp. nov.
该属（科： ；目： ；纲：Alphaproteobacteria 类）包括兼性细胞内革兰氏阴性、嗜血性、生长缓慢、媒介传播的细菌。野生啮齿动物及其跳蚤携带多种和株属，包括几种人畜共患病。这种遗传多样性加上生物体的挑剔性质导致了分类挑战，导致了大量未被描述的菌株的收集。在这里，我们报告了来自大鼠和跳蚤的两种菌株（即特拉维夫和 OE 1-1）的基因组和表型特征，这两种菌株属于属。扫描电子显微镜显示，这些细菌呈杆状，具有极性菌毛，长度在 1.0 到 2.0 μm 之间，宽度在 0.3 到 0.6 μm 之间。OE 1-1 和 Tel Aviv 菌株分别包含一个 2.16 和 2.23 Mbp 的单一染色体和一个 29.0 和 41.5 Kbp 的质粒，平均 DNA G+C 含量分别为 38.16 和 38.47 mol%。这些菌株的平均核苷酸同一性（ANI）为 89.9%。发现与这两个菌株最接近的系统发育相关物是 （ANI=90.9-93.6%）。在两个菌株中发现的主要脂肪酸是 Cω7、C16:0 和 C18:1。它们在 C14:0 和 C16:1 的组成上与 不同。两种菌株都是严格的嗜二氧化碳菌，它们的生化特征与具有有效发表名称的属的种相似，而芳基酰胺酶活性的差异部分有助于它们的分类。基因组和表型差异表明，OE 1-1 和 Tel Aviv 菌株代表了与 密切相关的新型单一物种，我们建议分别将它们命名为 sp. nov. 和 sp. nov.。
