Kešnerová Lucie, Moritz Roxane, Engel Philipp
Department of Fundamental Microbiology, University of Lausanne, CH-1015 Lausanne, Switzerland.
Int J Syst Evol Microbiol. 2016 Jan;66(1):414-421. doi: 10.1099/ijsem.0.000736. Epub 2015 Nov 3.
Here, we report the culture and characterization of an alphaproteobacterium of the order Rhizobiales, isolated from the gut of the honey bee Apis mellifera. Strain PEB0122T shares >95 % 16S rRNA gene sequence similarity with species of the genus Bartonella, a group of mammalian pathogens transmitted by bloodsucking arthropods. Phylogenetic analyses showed that PEB0122T and related strains from the honey bee gut form a sister clade of the genus Bartonella. Optimal growth of strain PEB0122T was obtained on solid media supplemented with defibrinated sheep blood under microaerophilic conditions at 35-37 °C, which is consistent with the cultural characteristics of other species of the genus Bartonella. Reduced growth of strain PEB0122T also occurred under aerobic conditions. The rod-shaped cells of strain PEB0122T had a mean length of 1.2-1.8 μm and revealed hairy surface structures. Strain PEB0122T was positive for catalase, cytochrome c oxidase, urease and nitrate reductase. The fatty acid composition was comparable to those of other species of the genus Bartonella, with palmitic acid (C16 : 0) and isomers of 18- and 19-carbon chains being the most abundant. The genomic DNA G+C content of PEB0122T was determined to be about 45.5 mol%. The high 16S rRNA gene sequence similarity with species of Bartonella and its close phylogenetic position suggest that strain PEB0122T represents a novel species within the genus Bartonella, for which we propose the name Bartonella apis sp. nov. The type strain is PEB0122T ( = NCIMB 14961T = DSM 29779T).
在此，我们报告了从蜜蜂Apis mellifera肠道中分离出的根瘤菌目α-变形菌的培养及特性鉴定。菌株PEB0122T与巴尔通体属的物种具有>95%的16S rRNA基因序列相似性，巴尔通体属是一组由吸血节肢动物传播的哺乳动物病原体。系统发育分析表明，PEB0122T及来自蜜蜂肠道的相关菌株形成了巴尔通体属的一个姐妹分支。菌株PEB0122T在补充有去纤维羊血的固体培养基上，于35 - 37°C微需氧条件下生长最佳，这与巴尔通体属其他物种的培养特性一致。在有氧条件下，菌株PEB0122T的生长也会减缓。菌株PEB0122T的杆状细胞平均长度为1.2 - 1.8μm，表面有毛发状结构。菌株PEB0122T的过氧化氢酶、细胞色素c氧化酶、脲酶和硝酸盐还原酶呈阳性。脂肪酸组成与巴尔通体属的其他物种相当，其中棕榈酸（C16 : 0）以及18碳和19碳链的异构体最为丰富。PEB0122T的基因组DNA G + C含量测定约为45.5mol%。与巴尔通体属物种的高16S rRNA基因序列相似性及其紧密的系统发育位置表明，菌株PEB0122T代表了巴尔通体属内的一个新物种，我们将其命名为Bartonella apis sp. nov.。模式菌株为PEB0122T（=NCIMB 14961T = DSM 29779T）。
