Key laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, PR China.
Chengdu Jinkai Bioengineering Co., Ltd, Chengdu 611130, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2217-2225. doi: 10.1099/ijsem.0.003964. Epub 2020 Feb 24.
A Gram-stain-negative, non-spore-forming, motile, aerobic, rod-shaped bacteria strain, designated LCB8, was isolated from the insect captured from a deserted cropland in Shuangliu district, Chengdu, PR China. Phylogenetic analysis on the basis of 16S rRNA gene sequence indicated that the strain represented a member of the genus , family , class CCUG 60088 (97.9 %) and CCUG 38531 (98.8 %) were identified as the most closely related phylogenetic neighbours of strain LCB8. The novel strain was able to grow at salt concentrations of 0-4.5 % (w/v), pH 5-9 and temperatures of 20-42 °C. The major quinone system was ubiquinone Q-10, the major fatty acids were Cω7, C and C. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and four undefined aminolipids. The major polyamines were putrescine and spermidine. Genome sequencing revealed a genome size of 4.76 Mbp and a DNA G+C content of 57.1 mol%. These phenotypic, genotypic and chemotaxonomic traits excellently supported the affiliation of LCB8 to the genus . Pairwise determined whole-genome average nucleotide identity (ANI) values indicated that strain LCB8 represents a novel species, for which we propose the name sp. nov. with the type strain LCB8 (=KCTC 72031=CGMCC 1.13984).
一株革兰氏阴性、非孢子形成、能动、需氧、杆状细菌菌株,被命名为 LCB8,从中国四川省成都市双流区废弃农田中捕获的昆虫中分离得到。基于 16S rRNA 基因序列的系统发育分析表明,该菌株代表了一个属的成员,与 CCUG 60088(97.9%)和 CCUG 38531(98.8%)最为密切相关。该新菌株能够在 0-4.5%(w/v)盐浓度、pH5-9 和 20-42°C 的温度下生长。主要醌类系统是泛醌 Q-10,主要脂肪酸是 Cω7、C 和 C。主要极性脂是磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂单甲醚乙醇胺、双磷脂酰甘油和四种未定义的氨基脂。主要多胺是腐胺和亚精胺。基因组测序显示基因组大小为 4.76 Mbp,DNA G+C 含量为 57.1mol%。这些表型、基因型和化学分类特征极好地支持了 LCB8 属于 属的归属。全基因组平均核苷酸同一性(ANI)值的成对测定表明,菌株 LCB8 代表了一个新的物种,我们建议使用 LCB8 株(=KCTC 72031=CGMCC 1.13984)作为其模式菌株,命名为 sp. nov.。