Penn D, Schmidt-Sommerfeld E
Zentrum für Kinderheilkunde, Justus Liebig Universitaet, Giessen, West Germany.
J Nutr. 1988 Dec;118(12):1535-9. doi: 10.1093/jn/118.12.1535.
This report describes the novel development of an animal model for neonatal carnitine deficiency using the artificially fed newborn rabbit. Each litter was separated from the mother following the first colostrum feeding and divided into 2 groups, one of which was fed a purified rabbit formula that was essentially free of carnitine; the other received the same formula supplemented with L-carnitine (100 mg/l). At 9-13 d of age, rabbit pups receiving the carnitine-free formula had lower concentrations of total, free and acylcarnitine in plasma and urine, as well as lower total acid soluble carnitine concentrations in liver, muscle, heart and brown adipose tissue than those receiving the same formula supplemented with L-carnitine. Their plasma and tissue levels were also lower, but their urinary carnitine concentrations were higher than those in naturally-raised pups. The findings suggest that the described animal model may prove to be a useful tool for the investigation of certain aspects of neonatal carnitine deficiency.
本报告描述了使用人工喂养的新生兔建立新生儿肉碱缺乏动物模型的新进展。每窝仔兔在首次喂食初乳后与母兔分开，分为两组，其中一组喂食基本不含肉碱的纯化兔奶粉；另一组接受添加了L-肉碱（100毫克/升）的相同奶粉。在9至13日龄时，接受不含肉碱奶粉的兔崽血浆和尿液中的总肉碱、游离肉碱和酰基肉碱浓度较低，肝脏、肌肉、心脏和棕色脂肪组织中的总酸溶性肉碱浓度也低于接受添加L-肉碱相同奶粉的兔崽。它们的血浆和组织水平也较低，但尿肉碱浓度高于自然饲养的幼崽。研究结果表明，所描述的动物模型可能被证明是研究新生儿肉碱缺乏某些方面的有用工具。
