Universidade Católica Portuguesa, Escola Superior de Biotecnologia, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associada, Rua de Diogo Botelho, 1327, 4169-005 Porto, Portugal.
Association BLC3 - Technology and Innovation Campus, Centre Bio R&D Unit, Senhora da Conceição, 2, Lagares, 3045-155 Oliveira do Hospital, Portugal.
Food Funct. 2020 Mar 26;11(3):2238-2254. doi: 10.1039/c9fo03000j.
Olive pomace is a semisolid by-product with great potential as a source of bioactive compounds. Using its soluble fraction, a liquid-enriched powder (LOPP) was obtained, exhibiting a rich composition in sugars, polyphenols and minerals, with potential antioxidant, antihypertensive and antidiabetic health benefits. To validate the potential of LOPP as a functional ingredient the effect of the gastrointestinal tract on its bioactive composition and bioactivities was examined. Polyphenols and minerals were the most affected compounds; however, a significant bioaccessibility of potassium and hydroxytyrosol was verified (≥57%). As a consequence, the LOPP bioactivities were only moderately affected (losses around 50%). For example, 57.82 ± 1.27% of the recovered antioxidant activity by ORAC was serum-available. From an initial α-glucosidase inhibition activity of 87.11 ± 1.04%, at least 50% of the initial potential was retained (43.82 ± 1.14%). Regarding the initial ACE inhibitory activity (91.98 ± 3.24%), after gastrointestinal tract losses, significant antihypertensive activity was retained in the serum-available fraction (43.4 ± 3.65%). The colon-available fraction also exhibited an abundant composition in phenolics and minerals. LOPP showed to be a potential functional ingredient not only with potential benefits in preventing cardiovascular diseases but also in gut health.
橄榄油渣是一种具有巨大潜力的半固体副产物，可作为生物活性化合物的来源。利用其可溶部分，获得了一种富含糖、多酚和矿物质的液体浓缩粉末（LOPP），具有潜在的抗氧化、降压和降血糖的健康益处。为了验证 LOPP 作为功能性成分的潜力，研究了胃肠道对其生物活性成分和生物活性的影响。多酚和矿物质是受影响最大的化合物；然而，钾和羟基酪醇的生物利用度得到了验证（≥57%）。因此，LOPP 的生物活性仅受到中度影响（损失约 50%）。例如，通过 ORAC 回收的抗氧化活性中有 57.82±1.27%可在血清中利用。从初始的α-葡萄糖苷酶抑制活性 87.11±1.04%，至少保留了 50%的初始潜力（43.82±1.14%）。对于初始的 ACE 抑制活性（91.98±3.24%），在胃肠道损失后，在可在血清中利用的部分保留了显著的降压活性（43.4±3.65%）。结肠可利用部分也含有丰富的酚类和矿物质。LOPP 不仅有望预防心血管疾病，而且对肠道健康也有潜在益处，是一种有潜力的功能性成分。
