Pereira Ricardo, Basto Ana, Pintado Manuela, Valente Luisa M P, Velasco Cristina
CIIMAR/CIMAR-LA, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal.
ICBAS, Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
Antioxidants (Basel). 2025 Mar 11;14(3):333. doi: 10.3390/antiox14030333.
This study investigates the effects of pineapple by-products on feed preservation during storage at two different temperatures (25 °C and 4 °C) and on European sea bass () stress resistance. Four isoproteic, isolipidic, and isoenergetic diets were manufactured: CTRL-negative control, commercial diet without added antioxidants; VITE-positive control, CTRL diet with 100 mg kg of vitamin E; and P2 and S2-VITE diet with 2% pineapple peel or stem flour, respectively. The fish (13.5 ± 0.8 g) were split into four replicate groups per diet and fed ad libitum for 12 weeks, after which they were subjected to a stress challenge of air exposure (1 min) followed by confinement (5 min, 100 kg m). Despite storage time lowering the antioxidant properties of all diets, P2 and S2 showed increased antioxidant capacity (DPPH, ABTS, and ORAC) before and after storage. The diets were well accepted by the fish, and the VITE-fed fish showed significantly lower lipid peroxidation values in the liver and muscle compared to all remaining diets. However, pineapple by-product inclusion did not result in increased fish stress resistance. Further optimization is required for the successful use of pineapple by-products as natural antioxidants in aquafeeds.
本研究调查了菠萝副产品在两种不同温度（25°C和4°C）下储存期间对饲料保存的影响以及对欧洲海鲈应激抗性的影响。制备了四种等蛋白、等脂质和等能量的饲料：CTRL-阴性对照，不添加抗氧化剂的商业饲料；VITE-阳性对照，添加100 mg/kg维生素E的CTRL饲料；以及分别添加2%菠萝皮粉或茎粉的P2和S2-VITE饲料。将鱼（13.5±0.8 g）按每种饲料分成四个重复组，随意投喂12周，之后对其进行空气暴露（1分钟）然后限制活动（5分钟，100 kg/m）的应激挑战。尽管储存时间降低了所有饲料的抗氧化性能，但P2和S2在储存前后均表现出抗氧化能力（DPPH、ABTS和ORAC）增强。这些饲料鱼都很爱吃，与所有其他饲料组相比，投喂VITE饲料的鱼肝脏和肌肉中的脂质过氧化值显著更低。然而，添加菠萝副产品并未提高鱼的应激抗性。要成功地将菠萝副产品用作水产饲料中的天然抗氧化剂，还需要进一步优化。
