Ahmad-Qasem Margarita H, Cánovas Jaime, Barrajón-Catalán Enrique, Carreres José E, Micol Vicente, García-Pérez José V
Grupo de Análisis y Simulación de Procesos Agroalimentarios (ASPA), Departamento de Tecnologı́a de Alimentos, Universitat Politècnica de València , Valencia 46022, Spain.
J Agric Food Chem. 2014 Jul 2;62(26):6190-8. doi: 10.1021/jf501414h. Epub 2014 Jun 24.
Olive leaves are rich in bioactive compounds, which are beneficial for humans. The objective of this work was to assess the influence of processing conditions (drying and extraction) of olive leaves on the extract's bioaccessibility. Thus, extracts obtained from dried olive leaves (hot air drying at 70 and 120 °C or freeze-drying) by means of conventional or ultrasound-assisted extraction were subjected to in vitro digestion. Antioxidant capacity, total phenolic content, and HPLC-DAD/MS/MS analysis were carried out during digestion. The dehydration treatment used for the olive leaves did not have a meaningful influence on bioaccessibility. The digestion process significantly (p<0.05) affected the composition of the extracts. Oleuropein and verbascoside were quite resistant to gastric digestion but were largely degraded in the intestinal phase. Nevertheless, luteolin-7-O-glucoside was the most stable polyphenol during the in vitro simulation (43% bioaccessibility). Therefore, this compound may be taken into consideration in further studies that focus on the bioactivity of olive leaf extracts.
橄榄叶富含生物活性化合物，对人体有益。本研究的目的是评估橄榄叶的加工条件（干燥和提取）对提取物生物可及性的影响。因此，通过常规或超声辅助提取从干燥橄榄叶（70℃和120℃热风干燥或冷冻干燥）中获得的提取物进行体外消化。在消化过程中进行抗氧化能力、总酚含量和HPLC-DAD/MS/MS分析。用于橄榄叶的脱水处理对生物可及性没有显著影响。消化过程显著（p<0.05）影响提取物的组成。橄榄苦苷和毛蕊花糖苷对胃消化相当耐受，但在肠道阶段大部分降解。然而，木犀草素-7-O-葡萄糖苷是体外模拟过程中最稳定的多酚（生物可及性为43%）。因此，在进一步关注橄榄叶提取物生物活性的研究中，可能需要考虑这种化合物。
