Calame K, Ihler G
Biochemistry. 1977 Mar 8;16(5):964-71. doi: 10.1021/bi00624a024.
A procedure is described which allows ribosomes bound to single-stranded DNA to be visualized in the electron microscope. The number of bound ribosomes may be determined and the position of the bound ribosomes may be readily measured along the DNA. The distribution of ribosomes bound to separated l and r strands of lambda DNA was shown to conform to the pattern predicted for binding at specific sites. The procedure should allow mapping of ribosome binding sites for the determination of genetic maps and may also be useful for studying translational control and relative binding affinities for ribosomes.
本文描述了一种方法，该方法可使结合在单链DNA上的核糖体在电子显微镜下可视化。可以确定结合的核糖体数量，并能很容易地沿着DNA测量结合核糖体的位置。已表明结合在λ噬菌体DNA的分离的l链和r链上的核糖体分布符合在特定位点结合所预测的模式。该方法应能绘制核糖体结合位点图谱以确定遗传图谱，也可能有助于研究翻译控制以及核糖体的相对结合亲和力。
