Thai Nguyen University of Sciences, Thai Nguyen City, Thai Nguyen Province 250000, Vietnam.
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2509-2514. doi: 10.1099/ijsem.0.004063. Epub 2020 Feb 26.
A Gram-stain-negative, aerobic and short rod-shaped bacterial strain, designated LD6, was isolated from a forest soil sample in Suwon, Gyeonggi-do, Republic of Korea. Strain LD6 grew at 10-37 °C (optimal temperature, 28 °C), and tolerated pH 8.0 and 2 % (w/v) NaCl. Strain LD6 was related most closely to members of the genus , namely NF2-5-3 (98.2 % 16S rRNA gene sequence similarity), A3 (97.9 %), DCY85 (97.9 %) and MWAP64 (97.7 %). The strain grew well on R2A agar, tryptone soya agar, Mueller-Hinton agar and nutrient agar. The major polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and glycolipid. The major respiratory quinone was ubiquinone 8 (Q-8). The main fatty acids were C cyclo, C, C 3-OH, C cyclo ω8 and C. The DNA G+C content of the isolated strain based on the whole genome sequence was 63.4 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain LD6 and its reference type strains ranged from 80.3 to 82.4%, and from 23.7 to 33.7%, respectively. Based on phenotypic, chemotypic and genotypic evidence, strain LD6 could be differentiated phylogenetically and phenotypically from the recognized species of the genus . Therefore, strain LD6 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is LD6 (=KACC 21387=JCM 33640).
一株革兰氏阴性、需氧、短杆状细菌菌株,被命名为 LD6,从韩国京畿道水原市的森林土壤样本中分离得到。菌株 LD6 在 10-37°C(最适温度 28°C)下生长,能耐受 pH8.0 和 2%(w/v)NaCl。菌株 LD6 与属内的成员最为密切相关,即 NF2-5-3(98.2% 16S rRNA 基因序列相似性)、A3(97.9%)、DCY85(97.9%)和 MWAP64(97.7%)。该菌株在 R2A 琼脂、胰蛋白胨大豆琼脂、Mueller-Hinton 琼脂和营养琼脂上生长良好。主要极性脂谱由磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、氨基磷脂和糖脂组成。主要呼吸醌为泛醌 8(Q-8)。主要脂肪酸为 C cyclo、C、C 3-OH、C cyclo ω8 和 C。基于全基因组序列的分离菌株的 DNA G+C 含量为 63.4mol%。菌株 LD6 与其参考型菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 80.3-82.4%和 23.7-33.7%。基于表型、化学型和基因型证据,菌株 LD6 在系统发育和表型上可与属内公认的种区分开来。因此,菌株 LD6 被认为代表一个新种,建议命名为 sp. nov.。模式菌株为 LD6(=KACC 21387=JCM 33640)。