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sp. nov.,分离自冷温带森林土壤。

sp. nov., isolated from cool temperate forest soil.

机构信息

Thai Nguyen University of Sciences, Thai Nguyen City, Thai Nguyen Province 250000, Vietnam.

Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2020 Apr;70(4):2509-2514. doi: 10.1099/ijsem.0.004063. Epub 2020 Feb 26.

Abstract

A Gram-stain-negative, aerobic and short rod-shaped bacterial strain, designated LD6, was isolated from a forest soil sample in Suwon, Gyeonggi-do, Republic of Korea. Strain LD6 grew at 10-37 °C (optimal temperature, 28 °C), and tolerated pH 8.0 and 2 % (w/v) NaCl. Strain LD6 was related most closely to members of the genus , namely NF2-5-3 (98.2 % 16S rRNA gene sequence similarity), A3 (97.9 %), DCY85 (97.9 %) and MWAP64 (97.7 %). The strain grew well on R2A agar, tryptone soya agar, Mueller-Hinton agar and nutrient agar. The major polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and glycolipid. The major respiratory quinone was ubiquinone 8 (Q-8). The main fatty acids were C cyclo, C, C 3-OH, C cyclo ω8 and C. The DNA G+C content of the isolated strain based on the whole genome sequence was 63.4 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain LD6 and its reference type strains ranged from 80.3 to 82.4%, and from 23.7 to 33.7%, respectively. Based on phenotypic, chemotypic and genotypic evidence, strain LD6 could be differentiated phylogenetically and phenotypically from the recognized species of the genus . Therefore, strain LD6 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is LD6 (=KACC 21387=JCM 33640).

摘要

一株革兰氏阴性、需氧、短杆状细菌菌株,被命名为 LD6,从韩国京畿道水原市的森林土壤样本中分离得到。菌株 LD6 在 10-37°C(最适温度 28°C)下生长,能耐受 pH8.0 和 2%(w/v)NaCl。菌株 LD6 与属内的成员最为密切相关,即 NF2-5-3(98.2% 16S rRNA 基因序列相似性)、A3(97.9%)、DCY85(97.9%)和 MWAP64(97.7%)。该菌株在 R2A 琼脂、胰蛋白胨大豆琼脂、Mueller-Hinton 琼脂和营养琼脂上生长良好。主要极性脂谱由磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、氨基磷脂和糖脂组成。主要呼吸醌为泛醌 8(Q-8)。主要脂肪酸为 C cyclo、C、C 3-OH、C cyclo ω8 和 C。基于全基因组序列的分离菌株的 DNA G+C 含量为 63.4mol%。菌株 LD6 与其参考型菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 80.3-82.4%和 23.7-33.7%。基于表型、化学型和基因型证据,菌株 LD6 在系统发育和表型上可与属内公认的种区分开来。因此,菌株 LD6 被认为代表一个新种,建议命名为 sp. nov.。模式菌株为 LD6(=KACC 21387=JCM 33640)。

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