State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2554-2559. doi: 10.1099/ijsem.0.004068. Epub 2020 Feb 26.
An orange-pigmented, short-rod-shaped, aerobic and non-motile bacterial strain, designated TK008, was isolated from the shallow-sea hydrothermal systems off Kueishantao Island in Taiwan, China, and it was studied by using a polyphasic taxonomic approach. Cells were Gram-stain-negative, and catalase- and oxidase-positive. Strain TK008 exhibited highest 16S rRNA gene sequence similarity of 97.1 % to F14. The phylogenetic trees based on 16S rRNA gene sequences showed that strain TK008 was a member of the genus . Digital DNA-DNA hybridization values between strain TK008 and two closely related species ( and ) were 20.6 and 20.9 %. The average nucleotide identity values of strain TK008 compared with and were 75.2 and 74.6 % respectively. The major isoprenoid quinone was ubiquinone-10. The predominant fatty acids (>10 %) were summed feature 8 (Cω6 and/or Cω7). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids, two unidentified lipids and an unidentified glycolipid. The DNA G+C content of strain TK008 from genomic sequence data was 62.54 mol%. On the basis of polyphasic analysis, strain TK008 represents a novel species, for which the name sp. nov. is proposed. The type strain is TK008 (=CGMCC 1.13898=JCM 33630).
一株橙色、短杆状、需氧且不运动的细菌菌株，被命名为 TK008，从中国台湾龟山岛附近的浅海热液系统中分离得到，通过多相分类学方法进行了研究。细胞革兰氏染色阴性，过氧化氢酶和氧化酶阳性。菌株 TK008 与 F14 的 16S rRNA 基因序列相似性最高，为 97.1%。基于 16S rRNA 基因序列构建的系统发育树显示，菌株 TK008 是 属的一个成员。菌株 TK008 与两个密切相关的种（和）之间的数字 DNA-DNA 杂交值分别为 20.6%和 20.9%。与和的平均核苷酸身份值分别为 75.2%和 74.6%。主要的类异戊二烯醌是泛醌-10。主要脂肪酸（>10%）为 summed feature 8（Cω6 和/或 Cω7）。极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、三种未鉴定的磷脂、两种未鉴定的脂和一种未鉴定的糖脂。基于基因组序列数据，菌株 TK008 的 DNA G+C 含量为 62.54mol%。基于多相分析，菌株 TK008 代表一个新种，建议命名为 sp. nov.。其模式菌株为 TK008（=CGMCC 1.13898=JCM 33630）。
