Departments of Pediatric Surgery.
Operation Room, The Affiliated Hospital of Qingdao University, Qingdao City, Shandong Province, PR. China.
Neuroreport. 2020 Mar 25;31(5):381-386. doi: 10.1097/WNR.0000000000001411.
Long non-coding (lncRNA) cancer susceptibility candidate (CASC7) plays a tumor-suppressive role in several malignancies. In this study, the role of CASC7 in neuroblastoma was investigated for the first time. We observed the downregulation of CASC7 in neuroblastoma tissues compared to non-cancer tissues of neuroblastoma patients. Across neuroblastoma tissues, CASC7 was inversely correlated with microRNA-10a (miR-10a) but positively correlated with phosphatase and tensin homolog mRNA. In neuroblastoma cells, CASC7 overexpression led to downregulated miR-10a but upregulated phosphatase and tensin homolog. Furthermore, miR-10a overexpression led to downregulated phosphatase and tensin homolog and reduced effects of CASC7 overexpression. CASC7 overexpression resulted in inhibition, while miR-10a overexpression resulted in increased proliferation rate of neuroblastoma cells. We therefore concluded that lncRNA CASC7 may upregulate phosphatase and tensin homolog by downregulating miR-10a to inhibit neuroblastoma cell proliferation.
长链非编码（lncRNA）癌症易感性候选物（CASC7）在几种恶性肿瘤中发挥肿瘤抑制作用。在这项研究中，首次研究了 CASC7 在神经母细胞瘤中的作用。与神经母细胞瘤患者的非癌组织相比，我们观察到 CASC7 在神经母细胞瘤组织中的下调。在神经母细胞瘤组织中，CASC7 与 microRNA-10a（miR-10a）呈负相关，但与磷酸酶和张力蛋白同源物 mRNA 呈正相关。在神经母细胞瘤细胞中，CASC7 的过表达导致 miR-10a 的下调，但磷酸酶和张力蛋白同源物的上调。此外，miR-10a 的过表达导致磷酸酶和张力蛋白同源物的下调，并降低了 CASC7 过表达的作用。CASC7 的过表达导致抑制，而 miR-10a 的过表达导致神经母细胞瘤细胞增殖率的增加。因此，我们得出结论，lncRNA CASC7 可能通过下调 miR-10a 来上调磷酸酶和张力蛋白同源物，从而抑制神经母细胞瘤细胞的增殖。
