Sun Wei, Wang Dao, Zu Yukun, Deng Yu
Department of Thoracic Surgery, Tongji Hospital, Huazhong University of Science and Technology, 430030, Wuhan, China.
Cell Death Discov. 2022 Apr 26;8(1):231. doi: 10.1038/s41420-022-01028-y.
Long noncoding RNAs have been proven to play a crucial role in many tumours. Here, we explored the role of the lncRNA cancer susceptibility candidate 7 (CASC7) in oesophageal cancer. LncRNA CASC7 was identified in our database analysis, and we found that it was significantly higher in oesophageal tumour tissue than in normal tissue and that high expression of lncRNA CASC7 predicted a poor prognosis. Furthermore, we verified through cell experiments that low expression of lncRNA CASC7 in oesophageal cancer cells significantly inhibited tumour proliferation, which could be explained by the effect of lncRNA CASC7 on aerobic glycolysis. Next, we found that the expression of CASC7 and hexokinase 2 (HK2) in oesophageal cancer was positively correlated in database analysis, and this conclusion was further verified in cell experiments. To determine the mechanism, we found that miR-143-3p can bind to both lncRNA CASC7 and HK2. In clinical specimens, we also found high expression of lncRNA CASC7 in tumours, and the expression levels of lncRNA CASC7 and HK2 were positively correlated. In conclusion, downregulating lncRNA CASC7 could inhibit tumour proliferation by reducing glycolysis through the miR-143-3p/HK2 axis.
长链非编码RNA已被证明在许多肿瘤中发挥关键作用。在此，我们探讨了长链非编码RNA癌症易感性候选基因7（CASC7）在食管癌中的作用。lncRNA CASC7是在我们的数据库分析中鉴定出来的，我们发现它在食管肿瘤组织中的表达明显高于正常组织，并且lncRNA CASC7的高表达预示着预后不良。此外，我们通过细胞实验证实，食管癌细胞中lncRNA CASC7的低表达显著抑制肿瘤增殖，这可以用lncRNA CASC7对有氧糖酵解的影响来解释。接下来，我们在数据库分析中发现食管癌中CASC7和己糖激酶2（HK2）的表达呈正相关，这一结论在细胞实验中得到了进一步验证。为了确定其机制，我们发现miR-143-3p可以同时与lncRNA CASC7和HK2结合。在临床标本中，我们还发现肿瘤中lncRNA CASC7高表达，并且lncRNA CASC7和HK2的表达水平呈正相关。总之，下调lncRNA CASC7可通过miR-143-3p/HK2轴减少糖酵解来抑制肿瘤增殖。
