Tian Liang, Huang Jiahe, Wen Aiyou, Yan Peishi
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China.
Animals (Basel). 2020 Feb 23;10(2):360. doi: 10.3390/ani10020360.
The aim of this study was to determine the effect of excessive back-fat (BF) of sows on placental oxidative stress, ATP generation, mitochondrial alterations in content and structure, and mitochondrial function in isolated trophoblasts. Placental tissue was collected by vaginal delivery from BFI (15-20 mm, n = 10) and BFII (21-27 mm, n = 10) sows formed according to BF at mating. Our results demonstrated that excessive back-fat contributed to augmented oxidative stress in term placenta, as evidenced by excessive production of ROS, elevated protein carbonylation, and reduced SOD, GSH-PX, and CAT activities ( < 0.05). Indicative of mitochondrial dysfunction, reduced mitochondrial respiration in cultured trophoblasts was linked to decreased ATP generation, lower mitochondrial Complex I activity and reduced expression of electron transport chain subunits in placenta of BFII sows ( < 0.05). Meanwhile, we observed negative alterations in mitochondrial biogenesis and structure in the placenta from BFII group ( < 0.05). Finally, our in vitro studies showed lipid-induced ROS production resulted in mitochondrial alterations in trophoblasts, and these effects were blocked by antioxidant treatment. Together, these data reveal that excessive back-fat aggravates mitochondrial injury induced by increased oxidative stress in pig term placenta, which may have detrimental consequences on placental function and therefore impaired fetal growth and development.
本研究的目的是确定母猪背部脂肪过多对胎盘氧化应激、ATP生成、线粒体含量和结构改变以及分离的滋养层细胞线粒体功能的影响。根据配种时的背部脂肪，将母猪分为BFI组（15 - 20毫米，n = 10）和BFII组（21 - 27毫米，n = 10），通过阴道分娩收集胎盘组织。我们的结果表明，过多的背部脂肪导致足月胎盘氧化应激增加，表现为活性氧过度产生、蛋白质羰基化升高以及超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性降低（< 0.05）。培养的滋养层细胞中线粒体呼吸减少表明线粒体功能障碍，这与BFII组母猪胎盘中ATP生成减少、线粒体复合体I活性降低以及电子传递链亚基表达减少有关（< 0.05）。同时，我们观察到BFII组胎盘线粒体生物合成和结构存在负面改变（< 0.05）。最后，我们的体外研究表明，脂质诱导的活性氧产生导致滋养层细胞线粒体改变，而抗氧化剂处理可阻断这些影响。总之，这些数据表明，过多的背部脂肪会加重猪足月胎盘氧化应激增加所诱导的线粒体损伤，这可能对胎盘功能产生不利影响，从而损害胎儿生长发育。
