Fu Yawei, Chen Yue, Jin Zhao, Gao Hu, Song Gang, Wang Qian, Xu Kang
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450000, China.
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
Antioxidants (Basel). 2022 May 25;11(6):1047. doi: 10.3390/antiox11061047.
Placental oxidative stress has been implicated as a main risk factor for placental dysfunction. Alleviation of oxidative stress and enhancement of antioxidant capacity of porcine trophectoderm (PTr2) cells are effective means to maintaining normal placental function. The present study was conducted to evaluate the protective effect of melatonin (MT) on HO-induced oxidative damage in PTr2 cells. Our data revealed that pretreatment with MT could significantly improve the decrease in cell viability induced by HO, and reduce intracellular reactive oxygen species (ROS) levels and the ratio of apoptotic cells. Here, we compared the transcriptomes of untreated versus melatonin-treated PTr2 cells by RNA-seq analysis and found that differentially expressed genes (DEGs) were highly enriched in the Wnt signaling, TGF-beta signaling and mTOR signaling pathways. Moreover, pretreatment with MT upregulated the antioxidant-related genes such as early growth response3 (EGR3), WAP four-disulfide core domain1 (WFDC1), heme oxygenase1 (HMOX1) and vimentin (VIM). These findings reveal that melatonin protects PTr2 cells from HO-induced oxidative stress damage.
胎盘氧化应激被认为是胎盘功能障碍的主要危险因素。减轻氧化应激并增强猪滋养外胚层（PTr2）细胞的抗氧化能力是维持正常胎盘功能的有效手段。本研究旨在评估褪黑素（MT）对HO诱导的PTr2细胞氧化损伤的保护作用。我们的数据显示，MT预处理可显著改善HO诱导的细胞活力下降，并降低细胞内活性氧（ROS）水平和凋亡细胞比例。在此，我们通过RNA测序分析比较了未处理与褪黑素处理的PTr2细胞的转录组，发现差异表达基因（DEG）在Wnt信号通路、TGF-β信号通路和mTOR信号通路中高度富集。此外，MT预处理上调了抗氧化相关基因，如早期生长反应3（EGR3）、富含WAP四二硫键核心结构域1（WFDC1）、血红素加氧酶1（HMOX1）和波形蛋白（VIM）。这些发现表明，褪黑素可保护PTr2细胞免受HO诱导的氧化应激损伤。
